Difference between revisions of "ThiT"

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=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
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=References=
 
=References=
 
==Reviews==
 
==Reviews==
<pubmed> 20497229 22574898</pubmed>
+
<pubmed> 20497229 22574898 24362466 </pubmed>
 
==Original publications==
 
==Original publications==
 
<pubmed>16291685, 21706007,18763711, 12376536 23602660</pubmed>
 
<pubmed>16291685, 21706007,18763711, 12376536 23602660</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 11:56, 10 January 2014

Gene name thiT
Synonyms yuaJ
Essential no
Product S protein of thiamine ECF transporter
Function thiamine uptake
Gene expression levels in SubtiExpress: thiT
Interactions involving this protein in SubtInteract: ThiT
Metabolic function and regulation of this protein in SubtiPathways:
thiT
MW, pI 20 kDa, 10.237
Gene length, protein length 576 bp, 192 aa
Immediate neighbours rrnB-16S, yuaI
Sequences Protein DNA DNA_with_flanks
Genetic context
YuaJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ThiT expression.png















Categories containing this gene/protein

ECF transporter, biosynthesis of cofactors, membrane proteins

This gene is a member of the following regulons

Thi-box

The gene

Basic information

  • Locus tag: BSU30990

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: proton-coupled thiamine transporter family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 3RLB (from Lactococcus lactis, 32% identity) PubMed
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications

Maria Majsnerowska, Inga Hänelt, Dorith Wunnicke, Lars V Schäfer, Heinz-Jürgen Steinhoff, Dirk Jan Slotboom
Substrate-induced conformational changes in the S-component ThiT from an energy coupling factor transporter.
Structure: 2013, 21(5);861-7
[PubMed:23602660] [WorldCat.org] [DOI] (I p)

Guus B Erkens, Ronnie P-A Berntsson, Faizah Fulyani, Maria Majsnerowska, Andreja Vujičić-Žagar, Josy Ter Beek, Bert Poolman, Dirk Jan Slotboom
The structural basis of modularity in ECF-type ABC transporters.
Nat Struct Mol Biol: 2011, 18(7);755-60
[PubMed:21706007] [WorldCat.org] [DOI] (I e)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Ghislain Schyns, Sébastien Potot, Yi Geng, Teresa M Barbosa, Adriano Henriques, John B Perkins
Isolation and characterization of new thiamine-deregulated mutants of Bacillus subtilis.
J Bacteriol: 2005, 187(23);8127-36
[PubMed:16291685] [WorldCat.org] [DOI] (P p)

Dmitry A Rodionov, Alexey G Vitreschak, Andrey A Mironov, Mikhail S Gelfand
Comparative genomics of thiamin biosynthesis in procaryotes. New genes and regulatory mechanisms.
J Biol Chem: 2002, 277(50);48949-59
[PubMed:12376536] [WorldCat.org] [DOI] (P p)