ThiM

• Description: hydroxyethylthiazole kinase
  
  

Gene name	thiM
Synonyms	thiK, ipa-25d, ywbJ
Essential	no
Product	hydroxyethylthiazole kinase
Function	biosynthesis of thiamine
Gene expression levels in SubtiExpress: thiM
Metabolic function and regulation of this protein in SubtiPathways: thiM
MW, pI	28 kDa, 5.393
Gene length, protein length	816 bp, 272 aa
Immediate neighbours	thiE, ywbI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

The gene

Basic information

• Locus tag: BSU38300

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole (according to Swiss-Prot)

• Protein family: Thz kinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ThiM-ThiM PubMed

• Localization:

Database entries

• Structure: 1C3Q

• UniProt: P39593

• KEGG entry: [3]

• E.C. number: 2.7.1.50

Expression and regulation

• Operon: ywbI-thiM-thiE PubMed

• Expression browser: thiM PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

Original publications

N Campobasso, I I Mathews, T P Begley, S E Ealick
Crystal structure of 4-methyl-5-beta-hydroxyethylthiazole kinase from Bacillus subtilis at 1.5 A resolution.
Biochemistry: 2000, 39(27);7868-77
[PubMed:10891066] [WorldCat.org] [DOI] (P p)

Y Zhang, S V Taylor, H J Chiu, T P Begley
Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis.
J Bacteriol: 1997, 179(9);3030-5
[PubMed:9139923] [WorldCat.org] [DOI] (P p)