Difference between revisions of "YwjH"

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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37110 ywjH]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37110 ywjH]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ywjH ywjH]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 22 kDa, 5.876   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 22 kDa, 5.876   

Revision as of 11:53, 7 January 2014

  • Description: transaldolase

Gene name ywjH
Synonyms
Essential no
Product transaldolase
Function pentose phosphate pathway
Gene expression levels in SubtiExpress: ywjH
Metabolic function and regulation of this protein in SubtiPathways:
ywjH
MW, pI 22 kDa, 5.876
Gene length, protein length 636 bp, 212 aa
Immediate neighbours murAB, fbaA
Sequences Protein DNA DNA_with_flanks
Genetic context
YwjH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YwjH expression.png




























Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU37110

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + D-fructose 6-phosphate (according to Swiss-Prot)
  • Protein family: Type 3B subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Ser-39 PubMed, in vitro phosphorylated by PrkC PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP819, available in Stülke lab
    • pGP1790 (expression of ywjH in B. subtilis, in pGP1389), available in Stülke lab
    • GP1409 (ywjH-Strep (spc)) & GP1411 (ywjH-Strep (cat)), purification from B. subtilis, for SPINE, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:
  • FLAG-tag construct: GP1407 (spc, based on pGP1331), available in the Stülke lab

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed