YloS

• Description: thiamine pyrophosphokinase
  
  

Gene name	yloS
Synonyms	thiN
Essential	no
Product	thiamine pyrophosphokinase
Function	thiamine salvage
Gene expression levels in SubtiExpress: yloS
Metabolic function and regulation of this protein in SubtiPathways: yloS
MW, pI	23 kDa, 5.216
Gene length, protein length	642 bp, 214 aa
Immediate neighbours	rpe, spoVM
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

The gene

Basic information

• Locus tag: BSU15800

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + thiamine = AMP + thiamine diphosphate (according to Swiss-Prot)

• Protein family: thiamine pyrophosphokinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3LM8

• UniProt: O34664

• KEGG entry: [2]

• E.C. number: 2.7.6.2

Expression and regulation

• Operon:

• Expression browser: yloS PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

Original publications

Ghislain Schyns, Sébastien Potot, Yi Geng, Teresa M Barbosa, Adriano Henriques, John B Perkins
Isolation and characterization of new thiamine-deregulated mutants of Bacillus subtilis.
J Bacteriol: 2005, 187(23);8127-36
[PubMed:16291685] [WorldCat.org] [DOI] (P p)

Jonathan Melnick, Ewa Lis, Joo-Heon Park, Cynthia Kinsland, Hirotada Mori, Tomoya Baba, John Perkins, Ghislain Schyns, Olga Vassieva, Andrei Osterman, Tadhg P Begley
Identification of the two missing bacterial genes involved in thiamine salvage: thiamine pyrophosphokinase and thiamine kinase.
J Bacteriol: 2004, 186(11);3660-2
[PubMed:15150256] [WorldCat.org] [DOI] (P p)