AnsZ

• Description: asparaginase
  
  

Gene name	ansZ
Synonyms	yccC
Essential	no
Product	asparaginase
Function	asparagine utilization
Gene expression levels in SubtiExpress: ansZ
Metabolic function and regulation of this protein in SubtiPathways: ansZ
MW, pI	39 kDa, 6.15
Gene length, protein length	1125 bp, 375 aa
Immediate neighbours	lmrA, lip
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

CodY regulon, TnrA regulon

The gene

Basic information

• Locus tag: BSU02690

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-asparagine + H₂O = L-aspartate + NH₃ (according to Swiss-Prot)

• Protein family: asparaginase 1 family (according to Swiss-Prot)

• Paralogous protein(s): AnsA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1ZCF (from Pectobacterium atrosepticum scri1043, 55% identity, 73% similarity)

• UniProt: O34482

• KEGG entry: [3]

• E.C. number: 3.5.1.1

Expression and regulation

• Operon: ansZ PubMed

• Expression browser: ansZ PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: