TilS

• Description: trigger enzyme, tRNAIle-lysidine synthetase (modifies C34 at the wobble position of tRNA2Ile to lysidine) and part of a transcription activator
  
  

Gene name	tilS
Synonyms	yacA
Essential	yes PubMed
Product	tRNAIle-lysidine synthetase
Function	tRNA modification, control of ftsH expression
Gene expression levels in SubtiExpress: tilS
Interactions involving this protein in SubtInteract: TilS
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation)
MW, pI	53 kDa, 8.317
Gene length, protein length	1416 bp, 472 aa
Immediate neighbours	yabT, hprT
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, transcription factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y), essential genes

This gene is a member of the following regulons

SigM regulon

The TilS-HprT regulon

• ftsH

The gene

Basic information

• Locus tag: BSU00670

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • modifies C34 at the wobble position of tRNA2Ile to lysidine

• Protein family: tRNA(Ile)-lysidine synthase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • TilS-HprT, the complex activates transcription of ftsH PubMed

• Localization:
  • Cytoplasm (Homogeneous) PubMed

Database entries

• Structure: 3A2K (from Geobacillus kaustophilus, complex with tRNA) PubMed

• UniProt: P37563

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: tilS-hprT-ftsH PubMed

• Expression browser: tilS PubMed

• Sigma factor: SigM PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Tsutomu Suzuki, Kenjyo Miyauchi
Discovery and characterization of tRNAIle lysidine synthetase (TilS).
FEBS Lett: 2010, 584(2);272-7
[PubMed:19944692] [WorldCat.org] [DOI] (I p)

Henri Grosjean, Glenn R Björk
Enzymatic conversion of cytidine to lysidine in anticodon of bacterial isoleucyl-tRNA--an alternative way of RNA editing.
Trends Biochem Sci: 2004, 29(4);165-8
[PubMed:15124629] [WorldCat.org] [DOI] (P p)