Difference between revisions of "DnaI"
| Line 37: | Line 37: | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
| − | |||
| − | |||
| − | |||
| − | |||
<br/><br/> | <br/><br/> | ||
| Line 102: | Line 98: | ||
=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2K7R 2K7R] (N-terminal domain) | + | * '''Structure:''' |
| + | ** [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2K7R 2K7R] (N-terminal domain) | ||
| + | ** [http://www.rcsb.org/pdb/explore.do?structureId=4M4W 4M4W] (the [[DnaC]]-[[DnaI]]-[[DnaG]] complex) {{PubMed|24048025}} | ||
* '''UniProt:''' [http://www.uniprot.org/uniprot/P06567 P06567] | * '''UniProt:''' [http://www.uniprot.org/uniprot/P06567 P06567] | ||
| Line 118: | Line 116: | ||
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=dnaI_2963185_2964120_-1 dnaI] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=dnaI_2963185_2964120_-1 dnaI] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
| Line 148: | Line 146: | ||
<pubmed> 22797751</pubmed> | <pubmed> 22797751</pubmed> | ||
==Original publications== | ==Original publications== | ||
| − | <pubmed>19255093,10844689,12718886,11842108, 20122408, 16479537, 19744498 , 19255093 19968790 11679082 </pubmed> | + | <pubmed>19255093,10844689,12718886,11842108, 20122408, 16479537, 19744498 , 19255093 19968790 11679082 24048025</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 08:32, 23 September 2013
- Description: primosome component (helicase loader), part of the replisome
| Gene name | dnaI |
| Synonyms | ytxA, dnaY |
| Essential | yes PubMed |
| Product | primosome component (helicase loader) |
| Function | DNA replication |
| Gene expression levels in SubtiExpress: dnaI | |
| Interactions involving this protein in SubtInteract: DnaI | |
| MW, pI | 35 kDa, 5.743 |
| Gene length, protein length | 933 bp, 311 aa |
| Immediate neighbours | ytxB, dnaB |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
DNA replication, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU28980
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot), Cytoplasm (Homogeneous) PubMed forms foci, close to oriC PubMed
Database entries
- UniProt: P06567
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
Bin Liu, William K Eliason, Thomas A Steitz
Structure of a helicase-helicase loader complex reveals insights into the mechanism of bacterial primosome assembly.
Nat Commun: 2013, 4;2495
[PubMed:24048025]
[WorldCat.org]
[DOI]
(I p)
Glenn M Sanders, H Garry Dallmann, Charles S McHenry
Reconstitution of the B. subtilis replisome with 13 proteins including two distinct replicases.
Mol Cell: 2010, 37(2);273-81
[PubMed:20122408]
[WorldCat.org]
[DOI]
(I p)
Wiep Klaas Smits, Alexi I Goranov, Alan D Grossman
Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo.
Mol Microbiol: 2010, 75(2);452-61
[PubMed:19968790]
[WorldCat.org]
[DOI]
(I p)
Kuang-Lei Tsai, Yu-Hua Lo, Yuh-Ju Sun, Chwan-Deng Hsiao
Molecular interplay between the replicative helicase DnaC and its loader protein DnaI from Geobacillus kaustophilus.
J Mol Biol: 2009, 393(5);1056-69
[PubMed:19744498]
[WorldCat.org]
[DOI]
(I p)
Karin V Loscha, Kristaps Jaudzems, Charikleia Ioannou, Xun-Cheng Su, Flynn R Hill, Gottfried Otting, Nicholas E Dixon, Edvards Liepinsh
A novel zinc-binding fold in the helicase interaction domain of the Bacillus subtilis DnaI helicase loader.
Nucleic Acids Res: 2009, 37(7);2395-404
[PubMed:19255093]
[WorldCat.org]
[DOI]
(I p)
Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537]
[WorldCat.org]
[DOI]
(P p)
Marion Velten, Stephen McGovern, Stéphanie Marsin, S Dusko Ehrlich, Philippe Noirot, Patrice Polard
A two-protein strategy for the functional loading of a cellular replicative DNA helicase.
Mol Cell: 2003, 11(4);1009-20
[PubMed:12718886]
[WorldCat.org]
[DOI]
(P p)
P Soultanas
A functional interaction between the putative primosomal protein DnaI and the main replicative DNA helicase DnaB in Bacillus.
Nucleic Acids Res: 2002, 30(4);966-74
[PubMed:11842108]
[WorldCat.org]
[DOI]
(I p)
C Bruand, M Farache, S McGovern, S D Ehrlich, P Polard
DnaB, DnaD and DnaI proteins are components of the Bacillus subtilis replication restart primosome.
Mol Microbiol: 2001, 42(1);245-55
[PubMed:11679082]
[WorldCat.org]
[DOI]
(P p)
Y Imai, N Ogasawara, D Ishigo-Oka, R Kadoya, T Daito, S Moriya
Subcellular localization of Dna-initiation proteins of Bacillus subtilis: evidence that chromosome replication begins at either edge of the nucleoids.
Mol Microbiol: 2000, 36(5);1037-48
[PubMed:10844689]
[WorldCat.org]
[DOI]
(P p)
