YfnI

• Description: polyglycerolphosphate lipoteichoic acid synthase, general stress protein, major secreted protein, required for survival at low temperature (4°C)
  
  

Gene name	yfnI
Synonyms	yetP, ltaSA
Essential	no
Product	minor lipoteichoic acid synthetase
Function	biosynthesis of lipoteichoic acid
Gene expression levels in SubtiExpress: yfnI
MW, pI	73 kDa, 5.951
Gene length, protein length	1917 bp, 639 aa
Immediate neighbours	yetO, yfnH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, cell envelope stress proteins (controlled by SigM, V, W, X, Y), phosphoproteins

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

• Locus tag: BSU07260

Phenotypes of a mutant

• induction of SigM and SigX activities PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: LTA synthase family (according to Swiss-Prot)

• Paralogous protein(s): LtaS, YqgS, YvgJ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on (Thr-311 OR Ser-312) PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • extracellular (signal peptide) PubMed

Database entries

• Structure:

• UniProt: Q797B3

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yfnI (according to DBTBS)

• Expression browser: yfnI PubMed

• Sigma factor: SigM PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • SM-NB (yfnI-spc), available in Anne Galinier's and Boris Görke's labs
  • GP1390 yfnI::spc, available in Jörg Stülke's lab
  • GP1397 yfnI::ermC, available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Additional reviews: PubMed

Nathalie T Reichmann, Angelika Gründling
Location, synthesis and function of glycolipids and polyglycerolphosphate lipoteichoic acid in Gram-positive bacteria of the phylum Firmicutes.
FEMS Microbiol Lett: 2011, 319(2);97-105
[PubMed:21388439] [WorldCat.org] [DOI] (I p)

Original publications

Additional publications: PubMed

Mirka E Wörmann, Rebecca M Corrigan, Peter J Simpson, Steve J Matthews, Angelika Gründling
Enzymatic activities and functional interdependencies of Bacillus subtilis lipoteichoic acid synthesis enzymes.
Mol Microbiol: 2011, 79(3);566-83
[PubMed:21255105] [WorldCat.org] [DOI] (I p)

Kathrin Schirner, Jon Marles-Wright, Richard J Lewis, Jeff Errington
Distinct and essential morphogenic functions for wall- and lipo-teichoic acids in Bacillus subtilis.
EMBO J: 2009, 28(7);830-42
[PubMed:19229300] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Adrian J Jervis, Penny D Thackray, Chris W Houston, Malcolm J Horsburgh, Anne Moir
SigM-responsive genes of Bacillus subtilis and their promoters.
J Bacteriol: 2007, 189(12);4534-8
[PubMed:17434969] [WorldCat.org] [DOI] (P p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)