Difference between revisions of "NarG"

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* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
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** part of a [[Respiratory complexes and supercomplexes|respiratory supercomplex]] {{PubMed|23880299}}
  
 
* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
** cell membrane (according to Swiss-Prot)
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** cell membrane {{PubMed|23880299}}
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=narG_3826259_3829945_-1 narG] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=narG_3826259_3829945_-1 narG] {{PubMed|22383849}}
  
* '''Sigma factor:'''  [[SigA]] {{PubMed|8846791,16428414}}
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* '''[[Sigma factor]]:'''  [[SigA]] {{PubMed|8846791,16428414}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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=References=
 
=References=
  
<pubmed>9352926,,8846791,16428414, </pubmed>
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<pubmed>9352926,23880299,8846791,16428414, </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 18:03, 11 August 2013

  • Description: nitrate reductase (alpha subunit)

Gene name narG
Synonyms
Essential no
Product nitrate reductase (alpha subunit)
Function nitrate respiration, nitrogen assimilation
Gene expression levels in SubtiExpress: narG
MW, pI 138 kDa, 6.061
Gene length, protein length 3684 bp, 1228 aa
Immediate neighbours narH, arfM
Sequences Protein DNA DNA_with_flanks
Genetic context
NarG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NarG expression.png















Categories containing this gene/protein

respiration, membrane proteins

This gene is a member of the following regulons

Fnr regulon

The gene

Basic information

  • Locus tag: BSU37280

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Nitrite + acceptor = nitrate + reduced acceptor (according to Swiss-Prot)
  • Protein family: prokaryotic molybdopterin-containing oxidoreductase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): contains an iron-sulfur cluster
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed under anaerobic conditions (Fnr) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Pedro M F Sousa, Marco A M Videira, Filipe A S Santos, Brian L Hood, Thomas P Conrads, Ana M P Melo
The bc:caa3 supercomplexes from the Gram positive bacterium Bacillus subtilis respiratory chain: a megacomplex organization?
Arch Biochem Biophys: 2013, 537(1);153-60
[PubMed:23880299] [WorldCat.org] [DOI] (I p)

Heike Reents, Richard Münch, Thorben Dammeyer, Dieter Jahn, Elisabeth Härtig
The Fnr regulon of Bacillus subtilis.
J Bacteriol: 2006, 188(3);1103-12
[PubMed:16428414] [WorldCat.org] [DOI] (P p)

M M Nakano, Y P Dailly, P Zuber, D P Clark
Characterization of anaerobic fermentative growth of Bacillus subtilis: identification of fermentation end products and genes required for growth.
J Bacteriol: 1997, 179(21);6749-55
[PubMed:9352926] [WorldCat.org] [DOI] (P p)

H Cruz Ramos, L Boursier, I Moszer, F Kunst, A Danchin, P Glaser
Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms.
EMBO J: 1995, 14(23);5984-94
[PubMed:8846791] [WorldCat.org] [DOI] (P p)