Difference between revisions of "Mbl"

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** [[MreB]]-[[Mbl]] {{PubMed|21636744}}
 
** [[MreB]]-[[Mbl]] {{PubMed|21636744}}
 
** [[Mbl]]-[[MreBH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17064365 PubMed]
 
** [[Mbl]]-[[MreBH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17064365 PubMed]
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** [[RodZ]]-[[Mbl]] {{PubMed|23879732}}
  
 
* '''[[Localization]]:'''  
 
* '''[[Localization]]:'''  
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mbl_3747254_3748255_-1 mbl] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mbl_3747254_3748255_-1 mbl] {{PubMed|22383849}}
  
* '''Sigma factor:''' [[SigE]] {{PubMed|9023218}}  
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* '''[[Sigma factor]]:''' [[SigE]] {{PubMed|9023218}}  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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<pubmed> 17064365,  20566861 21636744,21636745 22069484</pubmed>
 
<pubmed> 17064365,  20566861 21636744,21636745 22069484</pubmed>
 
==Other original publications==
 
==Other original publications==
<pubmed>19643765,16832063,11290328,12809607,16101995,12530960,7836311,,16950129 9023218, 19659933 19114499, 21091501 20525796 22048160 22343529</pubmed>
+
<pubmed>19643765,16832063,11290328,12809607,16101995,12530960,7836311,,16950129 9023218, 19659933 19114499, 21091501 20525796 22048160 22343529 23879732</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 08:20, 30 July 2013

  • Description: cell shape-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex

Gene name mbl
Synonyms
Essential no
Product MreB-like protein
Function cell shape determination
Gene expression levels in SubtiExpress: mbl
Interactions involving this protein in SubtInteract: Mbl
MW, pI 35 kDa, 5.669
Gene length, protein length 999 bp, 333 aa
Immediate neighbours flhO, spoIIID
Sequences Protein DNA DNA_with_flanks
Genetic context
Mbl context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Mbl expression.png















Categories containing this gene/protein

cell shape, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon, stringent response

The gene

Basic information

  • Locus tag: BSU36410

Phenotypes of a mutant

non-viable in the presence of low Mg(2+), readily accumulate rsgI suppressor mutants PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • forms helical filaments in a heterologous system PubMed
  • Protein family: ftsA/mreB family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • during logarithmic growth, Mbl forms discrete patches that move processively along peripheral tracks perpendicular to the cell axis PubMed
    • forms transverse bands as cells enter the stationary phase PubMed
    • close to the inner surface of the cytoplasmic membrane PubMed
    • reports on helical structures formed by Mbl PubMed seem to be misinterpretation of data PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed early during sporulation in the mother cell (SigE) PubMed
    • RelA dependent downregulation (Class I) during stringent response PubMed
    • expression is strongly repressed in the presence of aminoguanidine (inhibitor of nitric oxide synthase) PubMed
  • Regulatory mechanism:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage

Your additional remarks

References

Localization

Other original publications