Difference between revisions of "Pyk"
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pyk_2984788_2986545_-1 pyk] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pyk_2984788_2986545_-1 pyk] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
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* '''Mutant:''' | * '''Mutant:''' | ||
| − | * GP589 (''pyk''::''cat''), available in [[Stülke]] lab | + | * GP589 (''pyk''::''cat''), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}} |
| − | * GP600 (''pyk''::''erm''), available in [[Stülke]] lab | + | * GP600 (''pyk''::''erm''), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}} |
* '''Expression vector:''' | * '''Expression vector:''' | ||
| − | ** expression in ''E. coli'', N-terminal His-tag: pGP1100 (in [[pWH844]]), available in [[Stülke]] lab | + | ** expression in ''E. coli'', N-terminal His-tag: pGP1100 (in [[pWH844]]), available in [[Jörg Stülke]]'s lab |
| − | ** expression in ''B. subtilis'', native protein: pGP1411 (in [[pBQ200]]), available in [[Stülke]] lab | + | ** expression in ''B. subtilis'', native protein: pGP1411 (in [[pBQ200]]), available in [[Jörg Stülke]]'s lab |
| − | ** expression in ''B. subtilis'', N-terminal Strep-tag: pGP1409 (in [[pGP380]]), available in [[Stülke]] lab | + | ** expression in ''B. subtilis'', N-terminal Strep-tag: pGP1409 (in [[pGP380]]), available in [[Jörg Stülke]]'s lab |
| − | ** expression in ''B. subtilis'', C-terminal Strep-tag: pGP1410 (in [[pGP382]]), available in [[Stülke]] lab | + | ** expression in ''B. subtilis'', C-terminal Strep-tag: pGP1410 (in [[pGP382]]), available in [[Jörg Stülke]]'s lab |
* '''lacZ fusion:''' see ''[[pfkA]]'' | * '''lacZ fusion:''' see ''[[pfkA]]'' | ||
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* '''GFP fusion:''' | * '''GFP fusion:''' | ||
| − | * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab | + | * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab |
* '''Antibody:''' | * '''Antibody:''' | ||
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=References= | =References= | ||
| − | <pubmed>17726680 16493705 11489127 17505547 10966427 17218307 3711058 4623707 21821766 22846916 </pubmed> | + | <pubmed>17726680 16493705 11489127 17505547 10966427 17218307 3711058 4623707 21821766 22846916 23420519</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:02, 13 July 2013
- Description: pyruvate kinase, glycolytic enzyme
| Gene name | pyk |
| Synonyms | pykA |
| Essential | no |
| Product | pyruvate kinase |
| Function | catabolic enzyme in glycolysis |
| Gene expression levels in SubtiExpress: pyk | |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 61,9 kDa, 4.88 |
| Gene length, protein length | 1755 bp, 585 amino acids |
| Immediate neighbours | ytzA, pfkA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
ATP synthesis, carbon core metabolism, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU29180
Phenotypes of a mutant
Unable to grow with non-PTS carbohydrates (such as glucitol or glycerol) as single carbon source.
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
- A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ADP + phosphoenolpyruvate --> ATP + pyruvate
- The reaction is irreversible under physiological conditions
- Protein family: PEP-utilizing enzyme family (according to Swiss-Prot) pyruvate kinase family, (C-terminal section: PEP-utilizing enzyme family)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: Allosteric Regulation PubMed
- Domains:
- Modification: phosphorylation on Ser36 PubMed, PubMed, phosphorylation on Ser536 or Ser546 PubMed, please note that the Ser is not on position 536 but rather at 538
- Cofactor(s): Mg2+, K+
- Effectors of protein activity:
- Localization: cytoplasm PubMed
Database entries
- Structure: 2E28 (Geobacillus stearothermophilus)
- UniProt: P80885
- KEGG entry: [3]
- E.C. number: 2.7.1.40
Additional information
The enzyme is a tetramer with four active sites PubMed
Expression and regulation
- Regulation:
- twofold induced by glucose PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP589 (pyk::cat), available in Jörg Stülke's lab, PubMed
- GP600 (pyk::erm), available in Jörg Stülke's lab, PubMed
- Expression vector:
- expression in E. coli, N-terminal His-tag: pGP1100 (in pWH844), available in Jörg Stülke's lab
- expression in B. subtilis, native protein: pGP1411 (in pBQ200), available in Jörg Stülke's lab
- expression in B. subtilis, N-terminal Strep-tag: pGP1409 (in pGP380), available in Jörg Stülke's lab
- expression in B. subtilis, C-terminal Strep-tag: pGP1410 (in pGP382), available in Jörg Stülke's lab
- lacZ fusion: see pfkA
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Jörg Stülke, University of Göttingen, Germany Homepage
Your additional remarks
References
