Difference between revisions of "Pgk"
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* '''Mutant:''' | * '''Mutant:''' | ||
− | ** GP699 (''pgk''::''cat''), available in [[Stülke]] lab | + | ** GP699 (''pgk''::''cat''), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}}lab |
− | ** GP707 (''pgk''::''erm''), available in [[Stülke]] lab | + | ** GP707 (''pgk''::''erm''), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}} |
* '''Expression vector:''' | * '''Expression vector:''' | ||
− | ** pGP1102 (N-terminal His-tag, in [[pWH844]]), available in [[Stülke]] lab | + | ** pGP1102 (N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab |
− | ** pGP95 (N-terminal Strep-tag, in [[pGP172]]), available in [[Stülke]] lab | + | ** pGP95 (N-terminal Strep-tag, in [[pGP172]]), available in [[Jörg Stülke]]'s lab |
− | ** pGP91 (N-terminal Strep-tag, for [[SPINE]], expression in ''B. subtilis'', in [[pGP380]]), available in [[Stülke]] lab | + | ** pGP91 (N-terminal Strep-tag, for [[SPINE]], expression in ''B. subtilis'', in [[pGP380]]), available in [[Jörg Stülke]]'s lab |
− | ** pGP1513 (expression in ''B. subtilis'', in [[pBQ200]]), available in [[Stülke]] lab | + | ** pGP1513 (expression in ''B. subtilis'', in [[pBQ200]]), available in [[Jörg Stülke]]'s lab |
− | * '''lacZ fusion:''' pGP514 (in [[pAC6]]), a series of promoter deletion variants is also available in [[pAC6]], available in [[Stülke]] lab | + | * '''lacZ fusion:''' pGP514 (in [[pAC6]]), a series of promoter deletion variants is also available in [[pAC6]], available in [[Jörg Stülke]]'s lab |
* '''GFP fusion:''' | * '''GFP fusion:''' | ||
− | * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available | + | * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available iin [[Jörg Stülke]]'s lab |
* '''Antibody:''' | * '''Antibody:''' |
Revision as of 15:36, 13 July 2013
- Description: phosphoglycerate kinase, glycolytic/ gluconeogenic enzyme, universally conserved protein
Gene name | pgk |
Synonyms | |
Essential | No |
Product | phosphoglycerate kinase |
Function | enzyme in glycolysis/ gluconeogenesis |
Gene expression levels in SubtiExpress: pgk | |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
MW, pI | 42,0 kDa, 4.77 |
Gene length, protein length | 1182 bp, 394 amino acids |
Immediate neighbours | tpi, gapA |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
ATP synthesis, carbon core metabolism, phosphoproteins, universally conserved proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU33930
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + 3-phospho-D-glycerate = ADP + 1,3-bisphosphoglycerate (according to Swiss-Prot)
- Protein family: phosphoglycerate kinase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: Two Substrate Reversible Michaelis-Menten PubMed
- Domains:
- nucleotide binding domain (ATP) (350–353)
- 2x substrate binding domain (21–23), (59–62)
- Cofactor(s): Mg2+ or Mn2+ PubMed
- Effectors of protein activity:
- Inhibited by Co2+, NDP and NMP PubMed
- Localization: cytoplasm (according to Swiss-Prot), Cytoplasm (Homogeneous) PubMed
Database entries
- Structure: 1PHP (from Geobacillus stearothermophilus)
- UniProt: P40924
- KEGG entry: [3]
- E.C. number: 2.7.2.3
Additional information
- extensive information on the structure and enzymatic properties of Pgk can be found at Proteopedia
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- GP699 (pgk::cat), available in Jörg Stülke's lab, PubMedlab
- GP707 (pgk::erm), available in Jörg Stülke's lab, PubMed
- Expression vector:
- pGP1102 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
- pGP95 (N-terminal Strep-tag, in pGP172), available in Jörg Stülke's lab
- pGP91 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Jörg Stülke's lab
- pGP1513 (expression in B. subtilis, in pBQ200), available in Jörg Stülke's lab
- lacZ fusion: pGP514 (in pAC6), a series of promoter deletion variants is also available in pAC6, available in Jörg Stülke's lab
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available iin Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References