Difference between revisions of "Pgk"

From SubtiWiki
Jump to: navigation, search
Line 132: Line 132:
  
 
* '''Mutant:'''  
 
* '''Mutant:'''  
** GP699 (''pgk''::''cat''), available in [[Stülke]] lab
+
** GP699 (''pgk''::''cat''), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}}lab
** GP707 (''pgk''::''erm''), available in [[Stülke]] lab
+
** GP707 (''pgk''::''erm''), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}}
  
 
* '''Expression vector:'''  
 
* '''Expression vector:'''  
** pGP1102 (N-terminal His-tag, in [[pWH844]]), available in [[Stülke]] lab  
+
** pGP1102 (N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
** pGP95 (N-terminal Strep-tag, in [[pGP172]]), available in [[Stülke]] lab  
+
** pGP95 (N-terminal Strep-tag, in [[pGP172]]), available in [[Jörg Stülke]]'s lab
** pGP91 (N-terminal Strep-tag, for [[SPINE]], expression in ''B. subtilis'', in [[pGP380]]), available in [[Stülke]] lab  
+
** pGP91 (N-terminal Strep-tag, for [[SPINE]], expression in ''B. subtilis'', in [[pGP380]]), available in [[Jörg Stülke]]'s lab
** pGP1513 (expression in ''B. subtilis'', in [[pBQ200]]), available in [[Stülke]] lab
+
** pGP1513 (expression in ''B. subtilis'', in [[pBQ200]]), available in [[Jörg Stülke]]'s lab
 
 
* '''lacZ fusion:''' pGP514 (in [[pAC6]]), a series of promoter deletion variants is also available in [[pAC6]], available in [[Stülke]] lab
+
* '''lacZ fusion:''' pGP514 (in [[pAC6]]), a series of promoter deletion variants is also available in [[pAC6]], available in [[Jörg Stülke]]'s lab
  
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''
  
* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
+
* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available iin [[Jörg Stülke]]'s lab
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 15:36, 13 July 2013

Gene name pgk
Synonyms
Essential No
Product phosphoglycerate kinase
Function enzyme in glycolysis/ gluconeogenesis
Gene expression levels in SubtiExpress: pgk
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 42,0 kDa, 4.77
Gene length, protein length 1182 bp, 394 amino acids
Immediate neighbours tpi, gapA
Sequences Protein DNA DNA_with_flanks
Genetic context
Pgk context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Pgk expression.png















Categories containing this gene/protein

ATP synthesis, carbon core metabolism, phosphoproteins, universally conserved proteins

This gene is a member of the following regulons

CggR regulon

The gene

Basic information

  • Locus tag: BSU33930

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + 3-phospho-D-glycerate = ADP + 1,3-bisphosphoglycerate (according to Swiss-Prot)
  • Protein family: phosphoglycerate kinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: Two Substrate Reversible Michaelis-Menten PubMed
  • Domains:
    • nucleotide binding domain (ATP) (350–353)
    • 2x substrate binding domain (21–23), (59–62)
  • Modification: phosphorylation on Ser-183 AND Thr-299 PubMed, PubMed
  • Cofactor(s): Mg2+ or Mn2+ PubMed
  • Effectors of protein activity:
    • Inhibited by Co2+, NDP and NMP PubMed

Database entries

  • Structure: 1PHP (from Geobacillus stearothermophilus)
  • KEGG entry: [3]

Additional information

  • extensive information on the structure and enzymatic properties of Pgk can be found at Proteopedia

Expression and regulation

  • Regulatory mechanism: transcription repression by CggR PubMed
  • Additional information:

Biological materials

  • lacZ fusion: pGP514 (in pAC6), a series of promoter deletion variants is also available in pAC6, available in Jörg Stülke's lab
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available iin Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References