Difference between revisions of "MetC"

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Revision as of 13:00, 16 May 2013

  • Description: cystathionine beta-lyase

Gene name metC
Synonyms yjcJ
Essential no
Product cystathionine beta-lyase
Function biosynthesis of methionine
Gene expression levels in SubtiExpress: metC
Metabolic function and regulation of this protein in SubtiPathways:
Cys, Met & Sulfate assimilation
MW, pI 42 kDa, 5.757
Gene length, protein length 1170 bp, 390 aa
Immediate neighbours metI, yjcK
Sequences Protein DNA DNA_with_flanks
Genetic context
YjcJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MetC expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

S-box

The gene

Basic information

  • Locus tag: BSU11880

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-cystathionine + H2O = L-homocysteine + NH3 + pyruvate (according to Swiss-Prot)
  • Protein family: trans-sulfuration enzymes family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Jerneja Tomsic, Brooke A McDaniel, Frank J Grundy, Tina M Henkin
Natural variability in S-adenosylmethionine (SAM)-dependent riboswitches: S-box elements in bacillus subtilis exhibit differential sensitivity to SAM In vivo and in vitro.
J Bacteriol: 2008, 190(3);823-33
[PubMed:18039762] [WorldCat.org] [DOI] (I p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

Sandrine Auger, W H Yuen, Antoine Danchin, Isabelle Martin-Verstraete
The metIC operon involved in methionine biosynthesis in Bacillus subtilis is controlled by transcription antitermination.
Microbiology (Reading): 2002, 148(Pt 2);507-518
[PubMed:11832514] [WorldCat.org] [DOI] (P p)