RapF

• Description: response regulator aspartate phosphatase, anti-activator protein, controls ComA activity
  
  

Gene name	rapF
Synonyms	ywhJ
Essential	no
Product	response regulator aspartate phosphatase
Function	control of ComA activity
Gene expression levels in SubtiExpress: rapF
Interactions involving this protein in SubtInteract: RapF
MW, pI	45 kDa, 5.18
Gene length, protein length	1143 bp, 381 aa
Immediate neighbours	ywhK, phrF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, protein modification, transcription factors and their control, quorum sensing

This gene is a member of the following regulons

CcpA regulon, ComA regulon

The gene

Basic information

• Locus tag: BSU37460

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: control of ComA activity PubMed

• Protein family: RAP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: binding of PhrF to RapF results in a conformational change of RapF and prevents it from binding ComA PubMed

• Interactions:
  • RapF-ComA PubMed
  • RapF-PhrF PubMed

• Localization:

Database entries

• Structure:
  • 3ULQ (structure of the complex between the DNA-binding C-terminal domain of ComA with RapF) PubMed

• UniProt: P71002

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rapF-phrF PubMed

• Expression browser: rapF PubMed

• Sigma factor:

• Regulation: repressed by glucose (5-fold) (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • ComA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: