GlpX

• Description: class II fructose-1,6-bisphosphatase, gluconeogenesis
  
  

Gene name	glpX
Synonyms	ywjI
Essential	no
Product	class II fructose-1,6-bisphosphatase
Function	gluconeogenesis
Gene expression levels in SubtiExpress: glpX
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Sugar catabolism
MW, pI	33 kDa, 5.067
Gene length, protein length	963 bp, 321 aa
Immediate neighbours	rho, murAB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism

The gene

Basic information

• Locus tag: BSU37090

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-fructose 1,6-bisphosphate ==> D-fructose 6-phosphate + Pi

• Protein family: FBPase class 2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information: K(M) for FBP: 20 µM PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3D1R (from E. coli, 49% identity, 68% similarity)PubMed

• UniProt: Q03224

• KEGG entry: [3]

• E.C. number: 3.1.3.11

Expression and regulation

• Operon: murAB-glpX PubMed

• Expression browser: glpX PubMed

• Sigma factor:

• Regulation: constitutive PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Matthieu Jules, Ludovic Le Chat, Stéphane Aymerich, Dominique Le Coq
The Bacillus subtilis ywjI (glpX) gene encodes a class II fructose-1,6-bisphosphatase, functionally equivalent to the class III Fbp enzyme.
J Bacteriol: 2009, 191(9);3168-71
[PubMed:19270101] [WorldCat.org] [DOI] (I p)

Greg Brown, Alexander Singer, Vladimir V Lunin, Michael Proudfoot, Tatiana Skarina, Robert Flick, Samvel Kochinyan, Ruslan Sanishvili, Andrzej Joachimiak, Aled M Edwards, Alexei Savchenko, Alexander F Yakunin
Structural and biochemical characterization of the type II fructose-1,6-bisphosphatase GlpX from Escherichia coli.
J Biol Chem: 2009, 284(6);3784-92
[PubMed:19073594] [WorldCat.org] [DOI] (P p)