Difference between revisions of "CapC"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[capA]]'', ''[[capB]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[capA]]'', ''[[capB]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU35890 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU35890 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU35890 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU35890 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU35890 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU35890 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:ywtA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ywtA_context.gif]]

Revision as of 11:26, 14 May 2013

  • Description: capsular polyglutamate biosynthesis

Gene name capC
Synonyms ywtA, pgsC
Essential no
Product unknown
Function capsule synthesis
Gene expression levels in SubtiExpress: capC
MW, pI 16 kDa, 9.672
Gene length, protein length 447 bp, 149 aa
Immediate neighbours capA, capB
Sequences Protein DNA DNA_with_flanks
Genetic context
YwtA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CapC expression.png
























Categories containing this gene/protein

capsule biosynthesis and degradation

This gene is a member of the following regulons

DegU regulon

The gene

Basic information

  • Locus tag: BSU35890

Phenotypes of a mutant

no synthesis of the poly-gamma-glutamate capsule PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Additional information: The operon is not expressed in the lab strain 168 due to lack of expression of DegQ resulting in inactivity of DegS and DegU PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications

Additional publications: PubMed

Tohru Kamei, Daisuke Yamashiro, Terumi Horiuchii, Yutaka Minouchi, Makoto Ashiuchi
Identification and biochemical characterization of membranous short-chain polyglutamate from Bacillus subtilis.
Chem Biodivers: 2010, 7(6);1563-72
[PubMed:20564574] [WorldCat.org] [DOI] (I p)

Chuan-Mei Yeh, Jyh-Perng Wang, Shih-Ching Lo, Wen-Chia Chan, Ming-Yi Lin
Chromosomal integration of a synthetic expression control sequence achieves poly-gamma-glutamate production in a Bacillus subtilis strain.
Biotechnol Prog: 2010, 26(4);1001-7
[PubMed:20564357] [WorldCat.org] [DOI] (I p)

Keitarou Kimura, Lam-Son Phan Tran, Thi-Huyen Do, Yoshifumi Itoh
Expression of the pgsB encoding the poly-gamma-DL-glutamate synthetase of Bacillus subtilis (natto).
Biosci Biotechnol Biochem: 2009, 73(5);1149-55
[PubMed:19420703] [WorldCat.org] [DOI] (I p)

Yuji Urushibata, Shinji Tokuyama, Yasutaka Tahara
Difference in transcription levels of cap genes for gamma-polyglutamic acid production between Bacillus subtilis IFO 16449 and Marburg 168.
J Biosci Bioeng: 2002, 93(2);252-4
[PubMed:16233197] [WorldCat.org] [DOI] (P p)

Yuji Urushibata, Shinji Tokuyama, Yasutaka Tahara
Characterization of the Bacillus subtilis ywsC gene, involved in gamma-polyglutamic acid production.
J Bacteriol: 2002, 184(2);337-43
[PubMed:11751809] [WorldCat.org] [DOI] (P p)