RapE

• Description: response regulator aspartate phosphatase, dephosphorylates Spo0F-P, control of the phosphorelay
  
  

Gene name	rapE
Synonyms	yqcH
Essential	no
Product	response regulator aspartate phosphatase
Function	control of sporulation initiation
Gene expression levels in SubtiExpress: rapE
Interactions involving this protein in SubtInteract: RapE
Function and regulation of this protein in SubtiPathways: Phosphorelay
MW, pI	44 kDa, 7.313
Gene length, protein length	1125 bp, 375 aa
Immediate neighbours	yqcI, phrE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, transcription factors and their control, phosphorelay, quorum sensing, Skin element

This gene is a member of the following regulons

CodY regulon, ComA regulon

The gene

Basic information

• Locus tag: BSU25830

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: RAP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P45943

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rapE-phrE PubMed

• Expression browser: rapE PubMed

• Sigma factor:

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • ComA: transcription activation PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Boris R Belitsky, Abraham L Sonenshein
Genome-wide identification of Bacillus subtilis CodY-binding sites at single-nucleotide resolution.
Proc Natl Acad Sci U S A: 2013, 110(17);7026-31
[PubMed:23569278] [WorldCat.org] [DOI] (I p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

R S McQuade, N Comella, A D Grossman
Control of a family of phosphatase regulatory genes (phr) by the alternate sigma factor sigma-H of Bacillus subtilis.
J Bacteriol: 2001, 183(16);4905-9
[PubMed:11466295] [WorldCat.org] [DOI] (P p)

M Jiang, R Grau, M Perego
Differential processing of propeptide inhibitors of Rap phosphatases in Bacillus subtilis.
J Bacteriol: 2000, 182(2);303-10
[PubMed:10629174] [WorldCat.org] [DOI] (P p)