ResD

• Description: two-component response regulator, regulation of aerobic and anaerobic respiration
  
  

Gene name	resD
Synonyms	ypxD
Essential	no
Product	two-component response regulator
Function	regulation of aerobic and anaerobic respiration respiration
Gene expression levels in SubtiExpress: resD
Interactions involving this protein in SubtInteract: ResD
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation), Central C-metabolism, Stress, Alternative nitrogen sources
MW, pI	27 kDa, 5.631
Gene length, protein length	720 bp, 240 aa
Immediate neighbours	resE, resC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

regulators of electron transport, transcription factors and their control, membrane proteins, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, PhoP regulon, ResD regulon

The gene

Basic information

• Locus tag: BSU23120

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: OmpR family of two-component response regulators

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated by ResE on an Asp residue

• Cofactor(s):

• Effectors of protein activity: phosphorylation likely affects DNA-binding activity

• Interactions:
  • ResD interacts with the RNA polymerase PubMed
  • ResE-ResD

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P35163

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • resA-resB-resC-resD-resE PubMed
  • resD-resE PubMed

• Expression browser: resD PubMed

• Sigma factor:
  • resA: SigA PubMed
  • resD: SigA PubMed

• Regulation:
  • expressed under anaerobic conditions (ResD) PubMed
  • expressed under conditions of phosphate limitation (PhoP) PubMed
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • ResD: transcription activation PubMed
  • PhoP: transcription activation PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Marion Hulett, University of Illinois at Chicago, USA Homepage

References

Reviews

Elisabeth Härtig, Dieter Jahn
Regulation of the anaerobic metabolism in Bacillus subtilis.
Adv Microb Physiol: 2012, 61;195-216
[PubMed:23046954] [WorldCat.org] [DOI] (I p)

Original publications

Additional publications: PubMed

Sushma Kommineni, Amrita Lama, Benjamin Popescu, Michiko M Nakano
Global transcriptional control by NsrR in Bacillus subtilis.
J Bacteriol: 2012, 194(7);1679-88
[PubMed:22287527] [WorldCat.org] [DOI] (I p)

Sushma Kommineni, Erik Yukl, Takahiro Hayashi, Jacob Delepine, Hao Geng, Pierre Moënne-Loccoz, Michiko M Nakano
Nitric oxide-sensitive and -insensitive interaction of Bacillus subtilis NsrR with a ResDE-controlled promoter.
Mol Microbiol: 2010, 78(5);1280-93
[PubMed:21091510] [WorldCat.org] [DOI] (I p)

Ankita Puri-Taneja, Matthew Schau, Yinghua Chen, F Marion Hulett
Regulators of the Bacillus subtilis cydABCD operon: identification of a negative regulator, CcpA, and a positive regulator, ResD.
J Bacteriol: 2007, 189(9);3348-58
[PubMed:17322317] [WorldCat.org] [DOI] (P p)

Michiko M Nakano, Hao Geng, Shunji Nakano, Kazuo Kobayashi
The nitric oxide-responsive regulator NsrR controls ResDE-dependent gene expression.
J Bacteriol: 2006, 188(16);5878-87
[PubMed:16885456] [WorldCat.org] [DOI] (P p)

Elisabeth Härtig, Anja Hartmann, Manuela Schätzle, Alessandra M Albertini, Dieter Jahn
The Bacillus subtilis nrdEF genes, encoding a class Ib ribonucleotide reductase, are essential for aerobic and anaerobic growth.
Appl Environ Microbiol: 2006, 72(8);5260-5
[PubMed:16885274] [WorldCat.org] [DOI] (P p)

Soo-Keun Choi, Milton H Saier
Mechanism of CcpA-mediated glucose repression of the resABCDE operon of Bacillus subtilis.
J Mol Microbiol Biotechnol: 2006, 11(1-2);104-10
[PubMed:16825793] [WorldCat.org] [DOI] (P p)

Matthew Schau, Amr Eldakak, F Marion Hulett
Terminal oxidases are essential to bypass the requirement for ResD for full Pho induction in Bacillus subtilis.
J Bacteriol: 2004, 186(24);8424-32
[PubMed:15576792] [WorldCat.org] [DOI] (P p)

S Paul, X Zhang, F M Hulett
Two ResD-controlled promoters regulate ctaA expression in Bacillus subtilis.
J Bacteriol: 2001, 183(10);3237-46
[PubMed:11325953] [WorldCat.org] [DOI] (P p)

M M Nakano, Y Zhu
Involvement of ResE phosphatase activity in down-regulation of ResD-controlled genes in Bacillus subtilis during aerobic growth.
J Bacteriol: 2001, 183(6);1938-44
[PubMed:11222591] [WorldCat.org] [DOI] (P p)

X Zhang, F M Hulett
ResD signal transduction regulator of aerobic respiration in Bacillus subtilis: ctaA promoter regulation.
Mol Microbiol: 2000, 37(5);1208-19
[PubMed:10972837] [WorldCat.org] [DOI] (P p)

M M Nakano, Y Zhu, M Lacelle, X Zhang, F M Hulett
Interaction of ResD with regulatory regions of anaerobically induced genes in Bacillus subtilis.
Mol Microbiol: 2000, 37(5);1198-207
[PubMed:10972836] [WorldCat.org] [DOI] (P p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)

S M Birkey, W Liu, X Zhang, M F Duggan, F M Hulett
Pho signal transduction network reveals direct transcriptional regulation of one two-component system by another two-component regulator: Bacillus subtilis PhoP directly regulates production of ResD.
Mol Microbiol: 1998, 30(5);943-53
[PubMed:9988472] [WorldCat.org] [DOI] (P p)

M M Nakano, Y P Dailly, P Zuber, D P Clark
Characterization of anaerobic fermentative growth of Bacillus subtilis: identification of fermentation end products and genes required for growth.
J Bacteriol: 1997, 179(21);6749-55
[PubMed:9352926] [WorldCat.org] [DOI] (P p)

M M Nakano, P Zuber, P Glaser, A Danchin, F M Hulett
Two-component regulatory proteins ResD-ResE are required for transcriptional activation of fnr upon oxygen limitation in Bacillus subtilis.
J Bacteriol: 1996, 178(13);3796-802
[PubMed:8682783] [WorldCat.org] [DOI] (P p)

G Sun, E Sharkova, R Chesnut, S Birkey, M F Duggan, A Sorokin, P Pujic, S D Ehrlich, F M Hulett
Regulators of aerobic and anaerobic respiration in Bacillus subtilis.
J Bacteriol: 1996, 178(5);1374-85
[PubMed:8631715] [WorldCat.org] [DOI] (P p)