CysS

• Description: cysteine-tRNA synthetase
  
  

Gene name	cysS
Synonyms	snpA
Essential	yes PubMed
Product	cysteine-tRNA synthetase
Function	translation
Gene expression levels in SubtiExpress: cysS
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging, Cys, Met & Sulfate assimilation
MW, pI	53 kDa, 5.07
Gene length, protein length	1398 bp, 466 aa
Immediate neighbours	cysE, mrnC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU00940

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys) (according to Swiss-Prot)

• Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-270 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1U0B (from Escherichia coli, 42% identity, 56% similarity) PubMed

• UniProt: Q06752

• KEGG entry: [3]

• E.C. number: 6.1.1.16

Expression and regulation

• Operon: gltX-cysE-cysS-mrnC-yacO-yacP PubMed

• Expression browser: cysS PubMed

• Sigma factor: SigA PubMed

• Regulation: repression by cysteine (T-box) PubMed

• Regulatory mechanism: T-box, overlaps a transcription terminator upstream of cysE: transcription antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: