Difference between revisions of "QdoI"

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|style="background:#ABCDEF;" align="center"|'''Function''' || resistance to plant product quercetin
 
|style="background:#ABCDEF;" align="center"|'''Function''' || resistance to plant product quercetin
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU39980 qdoI]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU39980 qdoI]
 
|-
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37,4 kDa, 5.562   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37,4 kDa, 5.562   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yxaH]]'', ''[[qdoR]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yxaH]]'', ''[[qdoR]]''
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB16035&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU39980 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU39980 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU39980 Advanced_DNA]
 
|-
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yxaG_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yxaG_context.gif]]

Revision as of 14:17, 13 May 2013

  • Description: Fe-containing quercetin 2,3-dioxygenase

Gene name qdoI
Synonyms yxaG
Essential no
Product Fe-containing quercetin 2,3-dioxygenase
Function resistance to plant product quercetin
Gene expression levels in SubtiExpress: qdoI
MW, pI 37,4 kDa, 5.562
Gene length, protein length 1011 bp, 337 aa
Immediate neighbours yxaH, qdoR
Sequences Protein DNA Advanced_DNA
Genetic context
YxaG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
QdoI expression.png
























Categories containing this gene/protein

resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

This gene is a member of the following regulons

LmrA regulon, QdoR regulon

The gene

Basic information

  • Locus tag: BSU39980

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: oxidation of the flavonol quercetin with dioxygen, cleaving the central heterocyclic ring and releasing CO
  • Protein family:bicupin family
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): Mn(II) (preferred), but also active with Fe(II) snd Co(II) PubMed
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

J B Broderick
Catechol dioxygenases.
Essays Biochem: 1999, 34;173-89
[PubMed:10730195] [WorldCat.org] [DOI] (P p)

T D Bugg, J Sanvoisin, E L Spence
Exploring the catalytic mechanism of the extradiol catechol dioxygenases.
Biochem Soc Trans: 1997, 25(1);81-5
[PubMed:9056848] [WorldCat.org] [DOI] (P p)

Original publications