Difference between revisions of "ThiM"
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|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of thiamine | |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of thiamine | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU38300 thiM] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/thiamin.html Thiamin]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/thiamin.html Thiamin]''' | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[thiE]]'', ''[[ywbI]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[thiE]]'', ''[[ywbI]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU38300 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU38300 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU38300 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:thiM_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:thiM_context.gif]] | ||
Revision as of 14:10, 13 May 2013
- Description: hydroxyethylthiazole kinase
| Gene name | thiM |
| Synonyms | thiK, ipa-25d, ywbJ |
| Essential | no |
| Product | hydroxyethylthiazole kinase |
| Function | biosynthesis of thiamine |
| Gene expression levels in SubtiExpress: thiM | |
| Metabolic function and regulation of this protein in SubtiPathways: Thiamin | |
| MW, pI | 28 kDa, 5.393 |
| Gene length, protein length | 816 bp, 272 aa |
| Immediate neighbours | thiE, ywbI |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU38300
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole (according to Swiss-Prot)
- Protein family: Thz kinase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 1C3Q
- UniProt: P39593
- KEGG entry: [3]
- E.C. number: 2.7.1.50
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578]
[WorldCat.org]
[DOI]
(I p)
Original publications
N Campobasso, I I Mathews, T P Begley, S E Ealick
Crystal structure of 4-methyl-5-beta-hydroxyethylthiazole kinase from Bacillus subtilis at 1.5 A resolution.
Biochemistry: 2000, 39(27);7868-77
[PubMed:10891066]
[WorldCat.org]
[DOI]
(P p)
Y Zhang, S V Taylor, H J Chiu, T P Begley
Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis.
J Bacteriol: 1997, 179(9);3030-5
[PubMed:9139923]
[WorldCat.org]
[DOI]
(P p)
