Difference between revisions of "PyrG"
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|style="background:#ABCDEF;" align="center"|'''Function''' || pyrimidine biosynthesis | |style="background:#ABCDEF;" align="center"|'''Function''' || pyrimidine biosynthesis | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37150 pyrG] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/pyrimidines_metabolism.html Pyrimidines], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/pyrimidines_metabolism.html Pyrimidines], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]''' | ||
| Line 24: | Line 24: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywjG]]'', ''[[rpoE]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywjG]]'', ''[[rpoE]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37150 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37150 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37150 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:pyrG_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:pyrG_context.gif]] | ||
Revision as of 14:06, 13 May 2013
- Description: CTP synthase (NH3, glutamine)
| Gene name | pyrG |
| Synonyms | ctrA |
| Essential | yes PubMed |
| Product | CTP synthase (NH3, glutamine) |
| Function | pyrimidine biosynthesis |
| Gene expression levels in SubtiExpress: pyrG | |
| Metabolic function and regulation of this protein in SubtiPathways: Pyrimidines, Nucleotides (regulation) | |
| MW, pI | 59 kDa, 5.16 |
| Gene length, protein length | 1605 bp, 535 aa |
| Immediate neighbours | ywjG, rpoE |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of nucleotides, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU37150
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + UTP + NH3 = ADP + phosphate + CTP (according to Swiss-Prot)
- Protein family: CTP synthase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: P13242
- KEGG entry: [3]
- E.C. number: 6.3.4.2
Additional information
Expression and regulation
- Operon: pyrG PubMed
- Regulation:
- repressed by cytidine nucleotides (RNA switch) PubMed
- Regulatory mechanism:
- RNA switch: transcription (termination in the presence of cytidine nucleotides) PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Charles L Turnbough
Regulation of gene expression by reiterative transcription.
Curr Opin Microbiol: 2011, 14(2);142-7
[PubMed:21334966]
[WorldCat.org]
[DOI]
(I p)
Charles L Turnbough, Robert L Switzer
Regulation of pyrimidine biosynthetic gene expression in bacteria: repression without repressors.
Microbiol Mol Biol Rev: 2008, 72(2);266-300, table of contents
[PubMed:18535147]
[WorldCat.org]
[DOI]
(I p)
Original publications
Irene E Jensen-MacAllister, Qi Meng, Robert L Switzer
Regulation of pyrG expression in Bacillus subtilis: CTP-regulated antitermination and reiterative transcription with pyrG templates in vitro.
Mol Microbiol: 2007, 63(5);1440-52
[PubMed:17302819]
[WorldCat.org]
[DOI]
(P p)
Alexander K W Elsholz, Casper Møller Jørgensen, Robert L Switzer
The number of G residues in the Bacillus subtilis pyrG initially transcribed region governs reiterative transcription-mediated regulation.
J Bacteriol: 2007, 189(5);2176-80
[PubMed:17158658]
[WorldCat.org]
[DOI]
(P p)
Qi Meng, Charles L Turnbough, Robert L Switzer
Attenuation control of pyrG expression in Bacillus subtilis is mediated by CTP-sensitive reiterative transcription.
Proc Natl Acad Sci U S A: 2004, 101(30);10943-8
[PubMed:15252202]
[WorldCat.org]
[DOI]
(P p)
Qi Meng, Robert L Switzer
cis-acting sequences of Bacillus subtilis pyrG mRNA essential for regulation by antitermination.
J Bacteriol: 2002, 184(23);6734-8
[PubMed:12426364]
[WorldCat.org]
[DOI]
(P p)
Q Meng, R L Switzer
Regulation of transcription of the Bacillus subtilis pyrG gene, encoding cytidine triphosphate synthetase.
J Bacteriol: 2001, 183(19);5513-22
[PubMed:11544212]
[WorldCat.org]
[DOI]
(P p)
R J Turner, Y Lu, R L Switzer
Regulation of the Bacillus subtilis pyrimidine biosynthetic (pyr) gene cluster by an autogenous transcriptional attenuation mechanism.
J Bacteriol: 1994, 176(12);3708-22
[PubMed:8206849]
[WorldCat.org]
[DOI]
(P p)
C L Quinn, B T Stephenson, R L Switzer
Functional organization and nucleotide sequence of the Bacillus subtilis pyrimidine biosynthetic operon.
J Biol Chem: 1991, 266(14);9113-27
[PubMed:1709162]
[WorldCat.org]
(P p)
K Trach, J W Chapman, P Piggot, D LeCoq, J A Hoch
Complete sequence and transcriptional analysis of the spo0F region of the Bacillus subtilis chromosome.
J Bacteriol: 1988, 170(9);4194-208
[PubMed:2457578]
[WorldCat.org]
[DOI]
(P p)
