Difference between revisions of "YwjH"
(→Biological materials) |
|||
Line 14: | Line 14: | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || pentose phosphate pathway | |style="background:#ABCDEF;" align="center"|'''Function''' || pentose phosphate pathway | ||
|- | |- | ||
− | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37110 ywjH] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]''' | ||
Line 24: | Line 24: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[murAB]]'', ''[[fbaA]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[murAB]]'', ''[[fbaA]]'' | ||
|- | |- | ||
− | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37110 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37110 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37110 Advanced_DNA] |
|- | |- | ||
|- | |- |
Revision as of 14:05, 13 May 2013
- Description: transaldolase
Gene name | ywjH |
Synonyms | |
Essential | no |
Product | transaldolase |
Function | pentose phosphate pathway |
Gene expression levels in SubtiExpress: ywjH | |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
MW, pI | 22 kDa, 5.876 |
Gene length, protein length | 636 bp, 212 aa |
Immediate neighbours | murAB, fbaA |
Sequences | Protein DNA Advanced_DNA |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
carbon core metabolism, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU37110
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + D-fructose 6-phosphate (according to Swiss-Prot)
- Protein family: Type 3B subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- forms decamers (dimer of pentamers) PubMed
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- UniProt: P19669
- KEGG entry: [3]
- E.C. number: 2.2.1.2
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP819, available in Stülke lab
- pGP1790 (expression of ywjH in B. subtilis, in pGP1389), available in Stülke lab
- GP1409 (ywjH-Strep (spc)) & GP1411 (ywjH-Strep (cat)), purification from B. subtilis, for SPINE, available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed