Difference between revisions of "SsbB"

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|style="background:#ABCDEF;" align="center"|'''Function''' || required for efficient genetic transformation
 
|style="background:#ABCDEF;" align="center"|'''Function''' || required for efficient genetic transformation
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU36310 ssbB]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36310 ssbB]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SsbB SsbB]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SsbB SsbB]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glcR]]'', ''[[ywpG]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glcR]]'', ''[[ywpG]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15648&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36310 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36310 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36310 Advanced_DNA]
 
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Revision as of 14:02, 13 May 2013

  • Description: single-strand DNA-binding protein

Gene name ssbB
Synonyms ywpH
Essential no
Product single-strand DNA-binding protein
Function required for efficient genetic transformation
Gene expression levels in SubtiExpress: ssbB
Interactions involving this protein in SubtInteract: SsbB
MW, pI 12 kDa, 6.876
Gene length, protein length 339 bp, 113 aa
Immediate neighbours glcR, ywpG
Sequences Protein DNA Advanced_DNA
Genetic context
YwpH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SsbB expression.png















Categories containing this gene/protein

DNA replication, DNA repair/ recombination, phosphoproteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

  • Locus tag: BSU36310

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • binds and protects internalized foreign ssDNA PubMed
  • Protein family:
  • Paralogous protein(s): SsbA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: phosphorylation strongly increases DNA-binding activity PubMed
  • Localization:
    • localizes to cell poles in competent cells PubMed
    • localizes to the DNA entry pole during transformation PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed at the onset of stationary phase PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References