Difference between revisions of "CwlO"

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|style="background:#ABCDEF;" align="center"|'''Function''' || cell wall synthesis, cell proliferation
 
|style="background:#ABCDEF;" align="center"|'''Function''' || cell wall synthesis, cell proliferation
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU34800 cwlO]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34800 cwlO]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 50 kDa, 5.326   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 50 kDa, 5.326   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[trxB]]'', ''[[yvcD]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[trxB]]'', ''[[yvcD]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15485&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34800 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34800 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34800 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yvcE_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yvcE_context.gif]]

Revision as of 13:56, 13 May 2013

  • Description: D,L-endopeptidase-type autolysin

Gene name cwlO
Synonyms yzkA, yvcE
Essential no
Product endopeptidase-type autolysin
Function cell wall synthesis, cell proliferation
Gene expression levels in SubtiExpress: cwlO
MW, pI 50 kDa, 5.326
Gene length, protein length 1419 bp, 473 aa
Immediate neighbours trxB, yvcD
Sequences Protein DNA Advanced_DNA
Genetic context
YvcE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CwlO expression.png















Categories containing this gene/protein

cell wall degradation/ turnover

This gene is a member of the following regulons

WalR regulon

The gene

Basic information

  • Locus tag: BSU34800

Phenotypes of a mutant

a cwlO lytE mutant is not viable PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: peptidase C40 family (according to Swiss-Prot)
  • Paralogous protein(s): the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity

Extended information on the protein

  • Kinetic information:
  • Domains:
    • C-terminal D,L-endopeptidase domain PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • secreted (according to Swiss-Prot)
    • extracellular (signal peptide) PubMed
    • localizes to the lateral sidewall of the cell (via the N-terminal domain) PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulatory mechanism:
  • Additional information:
    • The mRNA has a long 5' leader region. This may indicate RNA-based regulation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Additional reviews: PubMed

Original publications

Additional publications: PubMed