KinB

• Description: two-component sensor kinase, phosphorylates Spo0F, part of the phosphorelay, senses changes in respiratory activity
  
  

Gene name	kinB
Synonyms
Essential	no
Product	two-component sensor kinase
Function	initiation of sporulation
Gene expression levels in SubtiExpress: kinB
Interactions involving this protein in SubtInteract: KinB
Function and regulation of this protein in SubtiPathways: Phosphorelay
MW, pI	47 kDa, 6.682
Gene length, protein length	1287 bp, 429 aa
Immediate neighbours	patB, kapB
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, transcription factors and their control, phosphorelay, membrane proteins, phosphoproteins

This gene is a member of the following regulons

CodY regulon, stringent response

The gene

Basic information

• Locus tag: BSU31450

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • autophosphorylation, phosphorylation of Spo0F
  • mainly active in the older, inner regions of a colony (with KinA) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: six transmembrane segments, C-terminal histidine phosphotransferase domain

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:
  • activity is triggered at low respiratory activity, this depends on a functional interaction with the respiration apparatus PubMed

• Interactions:
  • KinB-Sda
  • KinB-Spo0F
  • KinB-CtaC PubMed
  • KinB-QcrC PubMed

• Localization: cell membrane (integral membrane protein) PubMed

Database entries

• Structure: 3D36 (from G. stearothermophilus, complex with Sda)

• UniProt: Q08430

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: kinB-kapB PubMed

• Expression browser: kinB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • induced upon addition of decoyinine (positive stringent response) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: