Difference between revisions of "LeuS"
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|style="background:#ABCDEF;" align="center"|'''Function''' || translation | |style="background:#ABCDEF;" align="center"|'''Function''' || translation | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU30320 leuS] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/tRNA_charging.html tRNA charging]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/tRNA_charging.html tRNA charging]''' | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ytwF]]'', ''[[ytvB]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ytwF]]'', ''[[ytvB]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU30320 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU30320 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU30320 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:leuS_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:leuS_context.gif]] | ||
Revision as of 13:39, 13 May 2013
- Description: Leu-tRNA synthetase
| Gene name | leuS |
| Synonyms | |
| Essential | yes PubMed |
| Product | leucyl-tRNA synthetase |
| Function | translation |
| Gene expression levels in SubtiExpress: leuS | |
| Metabolic function and regulation of this protein in SubtiPathways: tRNA charging | |
| MW, pI | 91 kDa, 4.857 |
| Gene length, protein length | 2412 bp, 804 aa |
| Immediate neighbours | ytwF, ytvB |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU30320
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu) (according to Swiss-Prot)
- Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cytoplasm (according to Swiss-Prot)
Database entries
- UniProt: P36430
- KEGG entry: [3]
- E.C. number: 6.1.1.4
Additional information
Expression and regulation
- Sigma factor:
- Regulatory mechanism:
- T-box: RNA switch, binding of uncharged tRNA-Leu results in transcription antitermination PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532]
[WorldCat.org]
[DOI]
(I p)
Original Publications
P B Vander Horn, S A Zahler
Cloning and nucleotide sequence of the leucyl-tRNA synthetase gene of Bacillus subtilis.
J Bacteriol: 1992, 174(12);3928-35
[PubMed:1317842]
[WorldCat.org]
[DOI]
(P p)
