Difference between revisions of "NifS"

From SubtiWiki
Jump to: navigation, search
Line 14: Line 14:
 
|style="background:#ABCDEF;" align="center"|'''Function''' || NAD biosynthesis
 
|style="background:#ABCDEF;" align="center"|'''Function''' || NAD biosynthesis
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU27880 nifS]
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27880 nifS]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 6.425   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 6.425   
Line 22: Line 22:
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nadB]]'', ''[[nadR]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nadB]]'', ''[[nadR]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14693&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU27880 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU27880 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU27880 Advanced_DNA]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:nifS_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:nifS_context.gif]]

Revision as of 13:28, 13 May 2013

  • Description: cysteine desulfurase, required for NAD biosynthesis

Gene name nifS
Synonyms iscS
Essential no
Product cysteine desulfurase
Function NAD biosynthesis
Gene expression levels in SubtiExpress: nifS
MW, pI 43 kDa, 6.425
Gene length, protein length 1185 bp, 395 aa
Immediate neighbours nadB, nadR
Sequences Protein DNA Advanced_DNA
Genetic context
NifS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NifS expression.png
























Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

NadR regulon

The gene

Basic information

  • Locus tag: BSU27880

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine (according to Swiss-Prot)
  • Protein family: NifS/iscS subfamily (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed in the presence of nicotinic acid (NadR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References