Difference between revisions of "AnsB"

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|style="background:#ABCDEF;" align="center"|'''Function''' || aspartate degradation
 
|style="background:#ABCDEF;" align="center"|'''Function''' || aspartate degradation
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU23570 ansB]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23570 ansB]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/aspartate_asparagine.html Asp, Asn]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/aspartate_asparagine.html Asp, Asn]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mleN]]'', ''[[ansA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mleN]]'', ''[[ansA]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14289&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23570 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23570 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23570 Advanced_DNA]
 
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Revision as of 13:13, 13 May 2013

  • Description: L-aspartase

Gene name ansB
Synonyms
Essential no
Product L-aspartase
Function aspartate degradation
Gene expression levels in SubtiExpress: ansB
Metabolic function and regulation of this protein in SubtiPathways:
Asp, Asn
MW, pI 52 kDa, 5.69
Gene length, protein length 1425 bp, 475 aa
Immediate neighbours mleN, ansA
Sequences Protein DNA Advanced_DNA
Genetic context
AnsB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AnsB expression.png



















Categories containing this gene/protein

utilization of amino acids, membrane proteins

This gene is a member of the following regulons

AnsR regulon

The gene

Basic information

  • Locus tag: BSU23570

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-aspartate = fumarate + NH3 (according to Swiss-Prot)
  • Protein family: Aspartase subfamily (according to Swiss-Prot)
  • Paralogous protein(s): CitG

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3R6Q (from Bacillus sp. YM55-1, 72% identity, 92% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed in the presence of asparagine (AnsR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1153 (del ansAB::ermC) available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed