ResA

• Description: extracytoplasmic thioredoxin, cytochrome c biogenesis, reduces disulfide bonds in apo-cytochrome prior to the attachment of heme
  
  

Gene name	resA
Synonyms	ypxA
Essential	no
Product	extracytoplasmic thioredoxin
Function	cytochrome c biogenesis
Gene expression levels in SubtiExpress: resA
MW, pI	20 kDa, 8.37
Gene length, protein length	543 bp, 181 aa
Immediate neighbours	resB, rluB
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

respiration

This gene is a member of the following regulons

CcpA regulon, PhoP regulon, ResD regulon

The gene

Basic information

• Locus tag: BSU23150

Phenotypes of a mutant

• essential according to Kobayashi et al., but inactivation of resA was reported by Erlendsson et al.

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ResA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3C73

• UniProt: P35160

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: resA-resB-resC-resD-resE PubMed

• Expression browser: resA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under anaerobic conditions (ResD) PubMed
  • expressed under conditions of phosphate limitation (PhoP) PubMed
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • ResD: transcription activation PubMed
  • PhoP: transcription activation PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Christopher T C Hodson, Allison Lewin, Lars Hederstedt, Nick E Le Brun
The active-site cysteinyls and hydrophobic cavity residues of ResA are important for cytochrome c maturation in Bacillus subtilis.
J Bacteriol: 2008, 190(13);4697-705
[PubMed:18456809] [WorldCat.org] [DOI] (I p)

Allison Lewin, Allister Crow, Christopher T C Hodson, Lars Hederstedt, Nick E Le Brun
Effects of substitutions in the CXXC active-site motif of the extracytoplasmic thioredoxin ResA.
Biochem J: 2008, 414(1);81-91
[PubMed:18422485] [WorldCat.org] [DOI] (I p)

Allison Lewin, Allister Crow, Arthur Oubrie, Nick E Le Brun
Molecular basis for specificity of the extracytoplasmic thioredoxin ResA.
J Biol Chem: 2006, 281(46);35467-77
[PubMed:16971393] [WorldCat.org] [DOI] (P p)

Soo-Keun Choi, Milton H Saier
Mechanism of CcpA-mediated glucose repression of the resABCDE operon of Bacillus subtilis.
J Mol Microbiol Biotechnol: 2006, 11(1-2);104-10
[PubMed:16825793] [WorldCat.org] [DOI] (P p)

M M Nakano, Y Zhu
Involvement of ResE phosphatase activity in down-regulation of ResD-controlled genes in Bacillus subtilis during aerobic growth.
J Bacteriol: 2001, 183(6);1938-44
[PubMed:11222591] [WorldCat.org] [DOI] (P p)

S M Birkey, W Liu, X Zhang, M F Duggan, F M Hulett
Pho signal transduction network reveals direct transcriptional regulation of one two-component system by another two-component regulator: Bacillus subtilis PhoP directly regulates production of ResD.
Mol Microbiol: 1998, 30(5);943-53
[PubMed:9988472] [WorldCat.org] [DOI] (P p)

G Sun, E Sharkova, R Chesnut, S Birkey, M F Duggan, A Sorokin, P Pujic, S D Ehrlich, F M Hulett
Regulators of aerobic and anaerobic respiration in Bacillus subtilis.
J Bacteriol: 1996, 178(5);1374-85
[PubMed:8631715] [WorldCat.org] [DOI] (P p)

• Additional publications

PubMed