Difference between revisions of "LigB"

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|style="background:#ABCDEF;" align="center"|'''Function''' || phage SP-beta replication  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || phage SP-beta replication  
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU20500 ligB]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU20500 ligB]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 30 kDa, 6.865   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 30 kDa, 6.865   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yoqW]]'', ''[[yoqU]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yoqW]]'', ''[[yoqU]]''
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13942&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU20500 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU20500 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU20500 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:ligB_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ligB_context.gif]]

Revision as of 13:03, 13 May 2013

  • Description: DNA ligase (ATP-dependent)

Gene name ligB
Synonyms yoqV
Essential no
Product DNA ligase (ATP-dependent)
Function phage SP-beta replication
Gene expression levels in SubtiExpress: ligB
MW, pI 30 kDa, 6.865
Gene length, protein length 810 bp, 270 aa
Immediate neighbours yoqW, yoqU
Sequences Protein DNA Advanced_DNA
Genetic context
LigB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LigB expression.png
























Categories containing this gene/protein

DNA replication, SP-beta prophage

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU20500

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: peptidase M32 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

M A Petit, S D Ehrlich
The NAD-dependent ligase encoded by yerG is an essential gene of Bacillus subtilis.
Nucleic Acids Res: 2000, 28(23);4642-8
[PubMed:11095673] [WorldCat.org] [DOI] (I p)