Difference between revisions of "CheB"

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|style="background:#ABCDEF;" align="center"|'''Function''' || [[motility and chemotaxis]]
 
|style="background:#ABCDEF;" align="center"|'''Function''' || [[motility and chemotaxis]]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU16420 cheB]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16420 cheB]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CheB CheB]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CheB CheB]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[flhG]]'', ''[[cheA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[flhG]]'', ''[[cheA]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13515&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU16420 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU16420 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU16420 Advanced_DNA]
 
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Revision as of 12:51, 13 May 2013

  • Description: MCP-glutamate methylesterase / two-component response regulator-like

Gene name cheB
Synonyms cheL
Essential no
Product MCP-glutamate methylesterase / two-component response regulator-like
Function motility and chemotaxis
Gene expression levels in SubtiExpress: cheB
Interactions involving this protein in SubtInteract: CheB
MW, pI 38 kDa, 8.119
Gene length, protein length 1071 bp, 357 aa
Immediate neighbours flhG, cheA
Sequences Protein DNA Advanced_DNA
Genetic context
CheB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CheB expression.png















Categories containing this gene/protein

motility and chemotaxis, phosphoproteins

This gene is a member of the following regulons

CodY regulon, SigD regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU16420

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated on a Asp residue by CheA PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:
    • in minimal medium, CheB is present with 2,100 +/- 400 molecules per cell PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Gerald L Hazelbauer, Wing-Cheung Lai
Bacterial chemoreceptors: providing enhanced features to two-component signaling.
Curr Opin Microbiol: 2010, 13(2);124-32
[PubMed:20122866] [WorldCat.org] [DOI] (I p)

Original publications