Difference between revisions of "EcsA"
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|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of the secretion apparatus and of intra-membrane proteolysis | |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of the secretion apparatus and of intra-membrane proteolysis | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10040 ecsA] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/EcsA EcsA] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/EcsA EcsA] | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[hit]]'', ''[[ecsB]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[hit]]'', ''[[ecsB]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10040 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10040 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10040 Advanced_DNA] |
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|colspan="2" | '''Genetic context''' <br/> [[Image:ecsA_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:ecsA_context.gif]] | ||
Revision as of 12:28, 13 May 2013
- Description: ABC transporter (ATP-binding protein), mutation affects proteolysis by RasP (no processing of RsiW and FtsL)
| Gene name | ecsA |
| Synonyms | prsT, ecs-26, yhaD |
| Essential | no |
| Product | ABC transporter (ATP-binding protein) |
| Function | regulation of the secretion apparatus and of intra-membrane proteolysis |
| Gene expression levels in SubtiExpress: ecsA | |
| Interactions involving this protein in SubtInteract: EcsA | |
| MW, pI | 27 kDa, 5.635 |
| Gene length, protein length | 741 bp, 247 aa |
| Immediate neighbours | hit, ecsB |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
ABC transporters, protein secretion, sigma factors and their control, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU10040
Phenotypes of a mutant
no processing of RsiW and FtsL PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: ABC transporter domain (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: membrane associated (via EcsB) PubMed
Database entries
- Structure:
- UniProt: P55339
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information: the mRNA is very stable (half-life > 15 min) PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Janine Heinrich, Tuula Lundén, Vesa P Kontinen, Thomas Wiegert
The Bacillus subtilis ABC transporter EcsAB influences intramembrane proteolysis through RasP.
Microbiology (Reading): 2008, 154(Pt 7);1989-1997
[PubMed:18599827]
[WorldCat.org]
[DOI]
(P p)
G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008]
[WorldCat.org]
[DOI]
(P p)
Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453]
[WorldCat.org]
[DOI]
(P p)
S Leskelä, E Wahlström, H L Hyyryläinen, M Jacobs, A Palva, M Sarvas, V P Kontinen
Ecs, an ABC transporter of Bacillus subtilis: dual signal transduction functions affecting expression of secreted proteins as well as their secretion.
Mol Microbiol: 1999, 31(2);533-43
[PubMed:10027970]
[WorldCat.org]
[DOI]
(P p)
Soile Leskelä, Vesa P Kontinen, Matti Sarvas
Molecular analysis of an operon in Bacillus subtilis encoding a novel ABC transporter with a role in exoprotein production, sporulation and competence.
Microbiology (Reading): 1996, 142 ( Pt 1);71-77
[PubMed:8581172]
[WorldCat.org]
[DOI]
(P p)
