Difference between revisions of "LigA"
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|style="background:#ABCDEF;" align="center"|'''Function''' || replication | |style="background:#ABCDEF;" align="center"|'''Function''' || replication | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU06620 ligA] |
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 74 kDa, 4.708 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 74 kDa, 4.708 | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pcrA]]'', ''[[yerH]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pcrA]]'', ''[[yerH]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU06620 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU06620 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU06620 Advanced_DNA] |
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|colspan="2" | '''Genetic context''' <br/> [[Image:ligA_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:ligA_context.gif]] | ||
Revision as of 12:18, 13 May 2013
- Description: DNA ligase (NAD-dependent)
| Gene name | ligA |
| Synonyms | lig, yerG |
| Essential | yes PubMed |
| Product | DNA ligase (NAD-dependent) |
| Function | replication |
| Gene expression levels in SubtiExpress: ligA | |
| MW, pI | 74 kDa, 4.708 |
| Gene length, protein length | 2004 bp, 668 aa |
| Immediate neighbours | pcrA, yerH |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
DNA replication, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU06620
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m) (according to Swiss-Prot)
- Protein family: BRCT domain (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 1B04 (adenylation domain, Geobacillus stearothermophilus)
- UniProt: O31498
- KEGG entry: [3]
- E.C. number: 6.5.1.2
Additional information
Expression and regulation
- Sigma factor:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Nora Au, Elke Kuester-Schoeck, Veena Mandava, Laura E Bothwell, Susan P Canny, Karen Chachu, Sierra A Colavito, Shakierah N Fuller, Eli S Groban, Laura A Hensley, Theresa C O'Brien, Amish Shah, Jessica T Tierney, Louise L Tomm, Thomas M O'Gara, Alexi I Goranov, Alan D Grossman, Charles M Lovett
Genetic composition of the Bacillus subtilis SOS system.
J Bacteriol: 2005, 187(22);7655-66
[PubMed:16267290]
[WorldCat.org]
[DOI]
(P p)
M A Petit, S D Ehrlich
The NAD-dependent ligase encoded by yerG is an essential gene of Bacillus subtilis.
Nucleic Acids Res: 2000, 28(23);4642-8
[PubMed:11095673]
[WorldCat.org]
[DOI]
(I p)
M A Petit, E Dervyn, M Rose, K D Entian, S McGovern, S D Ehrlich, C Bruand
PcrA is an essential DNA helicase of Bacillus subtilis fulfilling functions both in repair and rolling-circle replication.
Mol Microbiol: 1998, 29(1);261-73
[PubMed:9701819]
[WorldCat.org]
[DOI]
(P p)
