Difference between revisions of "TsaD"
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|style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification | |style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU05940 tsaD] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/TsaD TsaD] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/TsaD TsaD] | ||
| Line 23: | Line 23: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ydiD]]'', ''[[ydiF]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ydiD]]'', ''[[ydiF]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU05940 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU05940 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU05940 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:gcp_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:gcp_context.gif]] | ||
Revision as of 12:15, 13 May 2013
- Description: required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA (together with TsaC), universally conserved protein
| Gene name | tsaD |
| Synonyms | ydiE, gcp |
| Essential | yes PubMed |
| Product | tRNA modification enzyme |
| Function | tRNA modification |
| Gene expression levels in SubtiExpress: tsaD | |
| Interactions involving this protein in SubtInteract: TsaD | |
| MW, pI | 36 kDa, 5.016 |
| Gene length, protein length | 1038 bp, 346 aa |
| Immediate neighbours | ydiD, ydiF |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
translation, essential genes, universally conserved proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU05940
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O05518
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Charles T Lauhon
Mechanism of N6-threonylcarbamoyladenonsine (t(6)A) biosynthesis: isolation and characterization of the intermediate threonylcarbamoyl-AMP.
Biochemistry: 2012, 51(44);8950-63
[PubMed:23072323]
[WorldCat.org]
[DOI]
(I p)
Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849]
[WorldCat.org]
[DOI]
(I p)
Publications on the corresponding protein from other species
Ting Lei, Junshu Yang, Li Zheng, Todd Markowski, Bruce A Witthuhn, Yinduo Ji
The essentiality of staphylococcal Gcp is independent of its repression of branched-chain amino acids biosynthesis.
PLoS One: 2012, 7(10);e46836
[PubMed:23056478]
[WorldCat.org]
[DOI]
(I p)
Ting Lei, Xudong Liang, Junshu Yang, Meiying Yan, Li Zheng, Bruce Walcheck, Yinduo Ji
The C-terminal domain of the novel essential protein Gcp is critical for interaction with another essential protein YeaZ of Staphylococcus aureus.
PLoS One: 2011, 6(5);e20163
[PubMed:21625506]
[WorldCat.org]
[DOI]
(I p)
Tobias Bergmiller, Rafael Peña-Miller, Alexander Boehm, Martin Ackermann
Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD.
BMC Microbiol: 2011, 11;118
[PubMed:21619589]
[WorldCat.org]
[DOI]
(I e)
Basma El Yacoubi, Isabelle Hatin, Christopher Deutsch, Tamer Kahveci, Jean-Pierre Rousset, Dirk Iwata-Reuyl, Alexey G Murzin, Valérie de Crécy-Lagard
A role for the universal Kae1/Qri7/YgjD (COG0533) family in tRNA modification.
EMBO J: 2011, 30(5);882-93
[PubMed:21285948]
[WorldCat.org]
[DOI]
(I p)
Madhusudhan Srinivasan, Preeti Mehta, Yao Yu, Evelyn Prugar, Eugene V Koonin, A Wali Karzai, Rolf Sternglanz
The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A.
EMBO J: 2011, 30(5);873-81
[PubMed:21183954]
[WorldCat.org]
[DOI]
(I p)
Chen Katz, Ifat Cohen-Or, Uri Gophna, Eliora Z Ron
The ubiquitous conserved glycopeptidase Gcp prevents accumulation of toxic glycated proteins.
mBio: 2010, 1(3);
[PubMed:20824107]
[WorldCat.org]
[DOI]
(I e)
Jennifer I Handford, Bérengère Ize, Grant Buchanan, Gareth P Butland, Jack Greenblatt, Andrew Emili, Tracy Palmer
Conserved network of proteins essential for bacterial viability.
J Bacteriol: 2009, 191(15);4732-49
[PubMed:19376873]
[WorldCat.org]
[DOI]
(I p)
