Difference between revisions of "PutB"

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(References)
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|style="background:#ABCDEF;" align="center"|'''Function''' || proline utilization
 
|style="background:#ABCDEF;" align="center"|'''Function''' || proline utilization
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU03200 putB]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03200 putB]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ycgL]]'', ''[[putC]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ycgL]]'', ''[[putC]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12114&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU03200 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU03200 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU03200 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:ycgM_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ycgM_context.gif]]

Revision as of 12:06, 13 May 2013

  • Description: proline dehydrogenase (L-proline, NAD)

Gene name putB
Synonyms ycgM
Essential no
Product proline dehydrogenase (L-proline, NAD)
Function proline utilization
Gene expression levels in SubtiExpress: putB
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 34 kDa, 7.291
Gene length, protein length 909 bp, 303 aa
Immediate neighbours ycgL, putC
Sequences Protein DNA Advanced_DNA
Genetic context
YcgM context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PutB expression.png



















Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

CodY regulon, PutR regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU03200

Phenotypes of a mutant

  • no growth with proline as single source of carbon or nitrogen PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor (according to Swiss-Prot)
  • Protein family: proline dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s): FadM

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced in the presence of proline (PutR) PubMed
    • expressed under conditions that trigger sporulation (Spo0A) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
    • strongly induced in response to glucose starvation in M9 medium PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed