Difference between revisions of "RapJ"
| Line 14: | Line 14: | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || control of the [[phosphorelay]] | |style="background:#ABCDEF;" align="center"|'''Function''' || control of the [[phosphorelay]] | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU02820 rapJ] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/RapJ RapJ] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/RapJ RapJ] | ||
| Line 24: | Line 24: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cwlK]]'', ''[[ycdF]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cwlK]]'', ''[[ycdF]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU02820 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU02820 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU02820 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:rapJ_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:rapJ_context.gif]] | ||
Revision as of 12:04, 13 May 2013
- Description: response regulator aspartate phosphatase, dephosphorylates Spo0F-P
| Gene name | rapJ |
| Synonyms | ycdE |
| Essential | no |
| Product | response regulator aspartate phosphatase |
| Function | control of the phosphorelay |
| Gene expression levels in SubtiExpress: rapJ | |
| Interactions involving this protein in SubtInteract: RapJ | |
| MW, pI | 44 kDa, 4.902 |
| Gene length, protein length | 1119 bp, 373 aa |
| Immediate neighbours | cwlK, ycdF |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
protein modification, phosphorelay, transcription factors and their control
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU02820
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Protein family: RAP family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- RapJ is made up of the C-terminal tetratricopeptide repeat (TPR) domain that is connected by a flexible helix containing linker to the N-terminal 3-helix bundle. Upon binding of the regulating peptide PhrC, the 3-helix bundle and the linker helix undergo a conformational change to form a TPR-like fold that merges with the existing C-terminal TPR domain. PubMed
- Modification:
- Cofactor(s):
Database entries
- UniProt: O34327
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: rapJ (according to DBTBS)
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Vijay Parashar, Philip D Jeffrey, Matthew B Neiditch
Conformational change-induced repeat domain expansion regulates Rap phosphatase quorum-sensing signal receptors.
PLoS Biol: 2013, 11(3);e1001512
[PubMed:23526881]
[WorldCat.org]
[DOI]
(I p)
Vijay Parashar, Nicolas Mirouze, David A Dubnau, Matthew B Neiditch
Structural basis of response regulator dephosphorylation by Rap phosphatases.
PLoS Biol: 2011, 9(2);e1000589
[PubMed:21346797]
[WorldCat.org]
[DOI]
(I e)
