YvcK

• Description: essential for growth under gluconeogenetic conditions, required for correct localization of PBP1
  
  

Gene name	yvcK
Synonyms
Essential	no
Product	unknown
Function	localization of PBP1
Gene expression levels in SubtiExpress: yvcK
MW, pI	34 kDa, 5.408
Gene length, protein length	951 bp, 317 aa
Immediate neighbours	yvcL, yvcJ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, cell shape

This gene is a member of the following regulons

Efp-dependent proteins

The gene

Basic information

• Locus tag: BSU34760

Phenotypes of a mutant

• unable to grow with gluconeogenic substrates as single carbon source PubMed
• filamentous or L-shape-like aberrant morphologies (suppressed by Mg++) PubMed, this is supressed by overexpression of MreB or by deletion of ponA (PBP1 ) PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: UPF0052 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: localized as a helical-like pattern PubMed

Database entries

• Structure:

• UniProt: O06974

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yvcI-yvcJ-yvcK-yvcL-crh-yvcN PubMed

• Expression browser: yvcK PubMed

• Sigma factor: SigA PubMed

• Regulation: very weak stimuation of expression by citrate and succinate PubMed

• Regulatory mechanism:

• Additional information:
  • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

• Mutant:

• Expression vector: pGP736 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab

• Antibody:

Labs working on this gene/protein

Boris Görke, University of Göttingen, Germany Homepage

Anne Galinier, University of Marseille, France

References

Reviews

PubMed