WapA

• Description: cell wall-associated protein precursor, contact-dependent growth inhibition protein
  
  

Gene name	wapA
Synonyms
Essential	no
Product	cell wall-associated protein precursor
Function	intercellular competition
Gene expression levels in SubtiExpress: wapA
Interactions involving this protein in SubtInteract: WapA
MW, pI	258 kDa, 6.131
Gene length, protein length	7002 bp, 2334 aa
Immediate neighbours	yxxG, yxxF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall/ other, toxins, antitoxins and immunity against toxins

This gene is a member of the following regulons

DegU regulon, YvrHb regulon

The gene

Basic information

• Locus tag: BSU39230

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relexed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • the C-terminal toxic domain has RNase activity (cleaves tRNAs) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • cell wall binding domain as retention signal
  • C-terminal toxic domain with RNase activity (cleaves tRNAs) PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • YxxG inhibits the toxic activity of WapA PubMed

• Interactions:
  • WapA-YxxG PubMed

• Localization:
  • extracellular (signal peptide), cell wall binding domain as retention signal, major constituent of the secretome PubMed

Database entries

• Structure:

• UniProt: Q07833

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: wapA-yxxG PubMed

• Expression browser: wapA PubMed

• Sigma factor:

• Regulation:
  • repressed at high salt concentration (DegU-P) PubMed

• Regulatory mechanism:
  • DegU-P: transcription repression PubMed
  • activated by YvrHb PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: