DprA

• Description: conveys incoming ssDNA to RecA
  
  

Gene name	dprA
Synonyms	smf
Essential	no
Product	recombination mediator protein
Function	DNA repair/ recombination
Gene expression levels in SubtiExpress: dprA
Interactions involving this protein in SubtInteract: DprA
MW, pI	32 kDa, 9.755
Gene length, protein length	891 bp, 297 aa
Immediate neighbours	sucD, topA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, genetic competence

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU16110

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • provides RecA access to ssDNA during chromosomal transformation (together with RecO) PubMed

• Protein family: smf family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DprA-RecA PubMed
  • DprA-DprA PubMed
  • DprA-ComFA PubMed
  • SsbB-DprA PubMed

• Localization:

Database entries

• Structure:

• UniProt: P39813

• KEGG entry: [3]

Expression and regulation

• Operon: dprA (according to DBTBS)

• Expression browser: dprA PubMed

• Sigma factor:

• Regulation:
  • expressed under conditions that trigger genetic competence (ComK) PubMed

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage

References

Nicolas Mirouze, Mathieu A Bergé, Anne-Lise Soulet, Isabelle Mortier-Barrière, Yves Quentin, Gwennaele Fichant, Chantal Granadel, Marie-Françoise Noirot-Gros, Philippe Noirot, Patrice Polard, Bernard Martin, Jean-Pierre Claverys
Direct involvement of DprA, the transformation-dedicated RecA loader, in the shut-off of pneumococcal competence.
Proc Natl Acad Sci U S A: 2013, 110(11);E1035-44
[PubMed:23440217] [WorldCat.org] [DOI] (I p)

Tribhuwan Yadav, Begoña Carrasco, Angela R Myers, Nicholas P George, James L Keck, Juan C Alonso
Genetic recombination in Bacillus subtilis: a division of labor between two single-strand DNA-binding proteins.
Nucleic Acids Res: 2012, 40(12);5546-59
[PubMed:22373918] [WorldCat.org] [DOI] (I p)

Serkalem Tadesse, Peter L Graumann
DprA/Smf protein localizes at the DNA uptake machinery in competent Bacillus subtilis cells.
BMC Microbiol: 2007, 7;105
[PubMed:18045469] [WorldCat.org] [DOI] (I e)

Isabelle Mortier-Barrière, Marion Velten, Pauline Dupaigne, Nicolas Mirouze, Olivier Piétrement, Stephen McGovern, Gwennaele Fichant, Bernard Martin, Philippe Noirot, Eric Le Cam, Patrice Polard, Jean-Pierre Claverys
A key presynaptic role in transformation for a widespread bacterial protein: DprA conveys incoming ssDNA to RecA.
Cell: 2007, 130(5);824-36
[PubMed:17803906] [WorldCat.org] [DOI] (P p)

Naomi Kramer, Jeanette Hahn, David Dubnau
Multiple interactions among the competence proteins of Bacillus subtilis.
Mol Microbiol: 2007, 65(2);454-64
[PubMed:17630974] [WorldCat.org] [DOI] (P p)

Janet C Lindow, Masayoshi Kuwano, Shigeki Moriya, Alan D Grossman
Subcellular localization of the Bacillus subtilis structural maintenance of chromosomes (SMC) protein.
Mol Microbiol: 2002, 46(4);997-1009
[PubMed:12421306] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Hirotake Yamaguchi, Kazuo Kobayashi, Naotake Ogasawara, Yasutaro Fujita, Teruo Tanaka
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.
J Bacteriol: 2002, 184(9);2344-51
[PubMed:11948146] [WorldCat.org] [DOI] (P p)