YkuT

• Description: mechanosensitive channel, similar to MscS, general stress protein
  
  

Gene name	ykuT
Synonyms
Essential	no
Product	mechanosensitive channel, similar to MscS
Function	resistance to osmotic downshock
Gene expression levels in SubtiExpress: ykuT
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	29 kDa, 5.65
Gene length, protein length	801 bp, 267 aa
Immediate neighbours	ykuS, ykuU
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

general stress proteins (controlled by SigB), coping with hypo-osmotic stress, membrane proteins

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

• Locus tag: BSU14210

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane PubMed

Database entries

• Structure:

• UniProt: O34897

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: ykuT PubMed

• Sigma factor: SigB PubMed

• Regulation: induced by stress (SigB) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A960 ( ykuT::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage

References

Reviews

Ian R Booth, Paul Blount
The MscS and MscL families of mechanosensitive channels act as microbial emergency release valves.
J Bacteriol: 2012, 194(18);4802-9
[PubMed:22685280] [WorldCat.org] [DOI] (I p)

Christopher D Pivetti, Ming-Ren Yen, Samantha Miller, Wolfgang Busch, Yi-Hsiung Tseng, Ian R Booth, Milton H Saier
Two families of mechanosensitive channel proteins.
Microbiol Mol Biol Rev: 2003, 67(1);66-85, table of contents
[PubMed:12626684] [WorldCat.org] [DOI] (P p)

Original Publications

Paul G Wahome, Ann E Cowan, Barbara Setlow, Peter Setlow
Levels and localization of mechanosensitive channel proteins in Bacillus subtilis.
Arch Microbiol: 2009, 191(5);403-14
[PubMed:19252899] [WorldCat.org] [DOI] (I p)

Tamara Hoffmann, Clara Boiangiu, Susanne Moses, Erhard Bremer
Responses of Bacillus subtilis to hypotonic challenges: physiological contributions of mechanosensitive channels to cellular survival.
Appl Environ Microbiol: 2008, 74(8);2454-60
[PubMed:18310427] [WorldCat.org] [DOI] (I p)

Paul G Wahome, Peter Setlow
Growth, osmotic downshock resistance and differentiation of Bacillus subtilis strains lacking mechanosensitive channels.
Arch Microbiol: 2008, 189(1);49-58
[PubMed:17665170] [WorldCat.org] [DOI] (P p)

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)