TsaC

• Description: L-threonylcarbamoyl-AMP synthase, biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A) (together with TsaB-TsaD-TsaE)
  
  

Gene name	ywlC
Synonyms	ipc-29d
Essential	no
Product	L-threonylcarbamoyl-AMP synthase
Function	tRNA modification
Gene expression levels in SubtiExpress: tsaC
MW, pI	36 kDa, 5.82
Gene length, protein length	1038 bp, 346 aa
Immediate neighbours	ywlD, ywlB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

This gene is a member of the following regulons

DnaA regulon

The gene

Basic information

• Locus tag: BSU36950

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • conversion of L-threonine, bicarbonate/CO2 and ATP to give the intermediate L-threonylcarbamoyl-AMP (TC-AMP) and pyrophosphate as products PubMed
  • biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A), a universal modification found at position 37 of ANN decoding tRNAs, which imparts a unique structure to the anticodon loop enhancing its binding to ribosomes in vitro (shown for the corresponding E. coli protein YrdC) PubMed

• Protein family: SUA5 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1HRU (the homolog from E. coli) PubMed

• UniProt: P39153

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: tsaC PubMed

• Expression browser: tsaC PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • DnaA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: