Pta

• Description: phosphotransacetylase
  
  

Gene name	pta
Synonyms	ipa-88d, ywfJ
Essential	no
Product	phosphotransacetylase
Function	overflow metabolism
Gene expression levels in SubtiExpress: pta
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	34 kDa, 4.649
Gene length, protein length	969 bp, 323 aa
Immediate neighbours	cysL, ywfI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

• Locus tag: BSU37660

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Acetyl-CoA + phosphate = CoA + acetyl phosphate (according to Swiss-Prot)

• Protein family: phosphate acetyltransferase and butyryltransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on (Ser-123 OR Thr-128 OR Ser-129) PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1XCO (complex with acetylphosphate), 1TD9

• UniProt: P39646

• KEGG entry: [3]

• E.C. number: 2.3.1.8

Expression and regulation

• Operon: pta PubMed

• Expression browser: pta PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expression activated by glucose (2.6 fold) (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: GP668 (aphA3), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538] [WorldCat.org] [DOI] (I e)