PutB

• Description: proline dehydrogenase (L-proline, NAD)
  
  

Gene name	putB
Synonyms	ycgM
Essential	no
Product	proline dehydrogenase (L-proline, NAD)
Function	proline utilization
Gene expression levels in SubtiExpress: putB
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	34 kDa, 7.291
Gene length, protein length	909 bp, 303 aa
Immediate neighbours	ycgL, putC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

CodY regulon, PutR regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU03200

Phenotypes of a mutant

• no growth with proline as single source of carbon or nitrogen PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor (according to Swiss-Prot)

• Protein family: proline dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s): FadM

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P94390

• KEGG entry: [3]

• E.C. number: 1.5.99.8

Expression and regulation

• Operon: putB-putC-putP 22139509 PubMed

• Expression browser: putB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced in the presence of proline (PutR) PubMed
  • expressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • strongly induced in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:
  • PutR: transcription activation PubMed
  • Spo0A: transcription activation PubMed
  • CodY: transcription repression, displacement of PutR PubMed

• Additional information:
  • overexpressed in a pnpA mutant PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed