GlnH

• Description: glutamine ABC transporter (binding protein)
  
  

Gene name	glnH
Synonyms
Essential	no
Product	glutamine ABC transporter (binding protein)
Function	glutamine uptake
Gene expression levels in SubtiExpress: glnH
Interactions involving this protein in SubtInteract: GlnH
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	29 kDa, 8.549
Gene length, protein length	819 bp, 273 aa
Immediate neighbours	glnQ, glnM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, biosynthesis/ acquisition of amino acids, glutamate metabolism, utilization of amino acids, membrane proteins, sporulation proteins

This gene is a member of the following regulons

SigE regulon, TnrA regulon

The gene

Basic information

• Locus tag: BSU27440

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:
  • K(D) for Gln: 120 myM PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GlnQ-(GlnM-GlnP)-GlnH PubMed

• Localization: attached to the membrane via GlnM-GlnP PubMed

Database entries

• Structure:

• UniProt: O34563

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: glnQ-glnH-glnM-glnP PubMed

• Expression browser: glnH PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • expressed during sporulation (SigE) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: GP734 (spc), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)