BglC

• Description: endo-1,4-beta-glucanase
  
  

Gene name	bglC
Synonyms	eglS
Essential	no
Product	endo-1,4-beta-glucanase)
Function	beta-1,4-glucan degradation
Gene expression levels in SubtiExpress: bglC
MW, pI	55 kDa, 8.619
Gene length, protein length	1497 bp, 499 aa
Immediate neighbours	alsT, ynfE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

The gene

Basic information

• Locus tag: BSU18130

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 5 (cellulase A) family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (signal peptide) PubMed

Database entries

• Structure: 3PZT PubMed

• UniProt: P10475

• KEGG entry: [3]

• E.C. number: 3.2.1.4

Expression and regulation

• Operon: bglC (according to DBTBS)

• Expression browser: bglC PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A751 (no resistance), PubMed, available at BGSC
  • 1A752 (no resistance), PubMed, available at BGSC
  • 1A976 (no resistance), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Camila R Santos, Joice H Paiva, Maurício L Sforça, Jorge L Neves, Rodrigo Z Navarro, Júnio Cota, Patrícia K Akao, Zaira B Hoffmam, Andréia N Meza, Juliana H Smetana, Maria L Nogueira, Igor Polikarpov, José Xavier-Neto, Fábio M Squina, Richard J Ward, Roberto Ruller, Ana C Zeri, Mário T Murakami
Dissecting structure-function-stability relationships of a thermostable GH5-CBM3 cellulase from Bacillus subtilis 168.
Biochem J: 2012, 441(1);95-104
[PubMed:21880019] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

L M Robson, G H Chambliss
Endo-beta-1,4-glucanase gene of Bacillus subtilis DLG.
J Bacteriol: 1987, 169(5);2017-25
[PubMed:3106328] [WorldCat.org] [DOI] (P p)

R M MacKay, A Lo, G Willick, M Zuker, S Baird, M Dove, F Moranelli, V Seligy
Structure of a Bacillus subtilis endo-beta-1,4-glucanase gene.
Nucleic Acids Res: 1986, 14(22);9159-70
[PubMed:3024130] [WorldCat.org] [DOI] (P p)