QueF

• Description: nitrile reductase (NADPH-dependent 7-cyano-7-deazaguanine reductase), synthesis of the modified ribonucleotide queuosine
  
  

Gene name	queF
Synonyms	ykvM
Essential	no
Product	nitrile reductase
Function	tRNA modification
Gene expression levels in SubtiExpress: queF
MW, pI	19 kDa, 4.927
Gene length, protein length	495 bp, 165 aa
Immediate neighbours	queE, ykvN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

This gene is a member of the following regulons

preQ1 riboswitch

The gene

Basic information

• Locus tag: BSU13750

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 7-aminomethyl-7-carbaguanine + 2 NADP⁺ = 7-cyano-7-carbaguanine + 2 NADPH (according to Swiss-Prot)

• Protein family: QueF type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: active site Cys56 is S-bacillithiolated by NaOCl stress PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 4F8B (covalent thioimide intermediate of the unimodular nitrile reductase QueF) PubMed

• UniProt: O31678

• KEGG entry: [2]

• E.C. number: 1.7.1.13

Expression and regulation

• Operon: queC-queD-queE-queF PubMed

• Expression browser: queF PubMed

• Sigma factor:

• Regulation:
  • repressed in the presence of queuosine (preQ1 riboswitch) PubMed

• Regulatory mechanism:
  • preQ1 riboswitch: transcriptional antitermination in the absence of queuosine PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: