Difference between revisions of "AlsS"
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Revision as of 08:27, 31 August 2012
- Description: acetolactate synthase
Gene name | alsS |
Synonyms | |
Essential | no |
Product | acetolactate synthase |
Function | overflow metabolism |
Gene expression levels in SubtiExpress: alsS | |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
MW, pI | 61 kDa, 5.164 |
Gene length, protein length | 1713 bp, 571 aa |
Immediate neighbours | alsD, alsR |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
AlsR regulon, Rex regulon, stringent response
The gene
Basic information
- Locus tag: BSU36010
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 2 pyruvate = 2-acetolactate + CO2 (according to Swiss-Prot)
- Protein family: TPP enzyme family (according to Swiss-Prot)
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: Q04789
- KEGG entry: [3]
- E.C. number: 2.2.1.6
Additional information
Expression and regulation
- Regulation:
Note: since acetate formation requires ackA activation by CcpA there is an indirect effect of CcpA on the alsSD operon: the operon is not expressed in ccpA mutants
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
Shigeo Tojo, Kanako Kumamoto, Kazutake Hirooka, Yasutaro Fujita
Heavy involvement of stringent transcription control depending on the adenine or guanine species of the transcription initiation site in glucose and pyruvate metabolism in Bacillus subtilis.
J Bacteriol: 2010, 192(6);1573-85
[PubMed:20081037]
[WorldCat.org]
[DOI]
(I p)
Shota Atsumi, Zhen Li, James C Liao
Acetolactate synthase from Bacillus subtilis serves as a 2-ketoisovalerate decarboxylase for isobutanol biosynthesis in Escherichia coli.
Appl Environ Microbiol: 2009, 75(19);6306-11
[PubMed:19684168]
[WorldCat.org]
[DOI]
(I p)
Susana Romero-Garcia, Claudia Hernández-Bustos, Enrique Merino, Guillermo Gosset, Alfredo Martinez
Homolactic fermentation from glucose and cellobiose using Bacillus subtilis.
Microb Cell Fact: 2009, 8;23
[PubMed:19383131]
[WorldCat.org]
[DOI]
(I e)
Takashi Inaoka, Takenori Satomura, Yasutaro Fujita, Kozo Ochi
Novel gene regulation mediated by overproduction of secondary metabolite neotrehalosadiamine in Bacillus subtilis.
FEMS Microbiol Lett: 2009, 291(2);151-6
[PubMed:19087206]
[WorldCat.org]
[DOI]
(I p)
Heike Reents, Richard Münch, Thorben Dammeyer, Dieter Jahn, Elisabeth Härtig
The Fnr regulon of Bacillus subtilis.
J Bacteriol: 2006, 188(3);1103-12
[PubMed:16428414]
[WorldCat.org]
[DOI]
(P p)
A J Turinsky, T R Moir-Blais, F J Grundy, T M Henkin
Bacillus subtilis ccpA gene mutants specifically defective in activation of acetoin biosynthesis.
J Bacteriol: 2000, 182(19);5611-4
[PubMed:10986270]
[WorldCat.org]
[DOI]
(P p)
M C Renna, N Najimudin, L R Winik, S A Zahler
Regulation of the Bacillus subtilis alsS, alsD, and alsR genes involved in post-exponential-phase production of acetoin.
J Bacteriol: 1993, 175(12);3863-75
[PubMed:7685336]
[WorldCat.org]
[DOI]
(P p)