NudF

• Description: involved in isopentenol (isoprenoid) biosynthesis
  
  

Gene name	nudF
Synonyms	yqkG
Essential	no
Product	unknown
Function	isoprenoid biosynthesis
Gene expression levels in SubtiExpress: nudF
MW, pI	20 kDa, 4.586
Gene length, protein length	555 bp, 185 aa
Immediate neighbours	yqxK, mciZ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of lipids

The gene

Basic information

• Locus tag: BSU23610

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ADP-ribose + H₂O = AMP + D-ribose 5-phosphate (according to Swiss-Prot)

• Protein family: Nudix hydrolase, NudF subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P54570

• KEGG entry: [2]

• E.C. number: 3.6.1.13

Expression and regulation

• Operon:

• Expression browser: nudF PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Sydnor T Withers, Shayin S Gottlieb, Bonny Lieu, Jack D Newman, Jay D Keasling
Identification of isopentenol biosynthetic genes from Bacillus subtilis by a screening method based on isoprenoid precursor toxicity.
Appl Environ Microbiol: 2007, 73(19);6277-83
[PubMed:17693564] [WorldCat.org] [DOI] (P p)

C A Dunn, S F O'Handley, D N Frick, M J Bessman
Studies on the ADP-ribose pyrophosphatase subfamily of the nudix hydrolases and tentative identification of trgB, a gene associated with tellurite resistance.
J Biol Chem: 1999, 274(45);32318-24
[PubMed:10542272] [WorldCat.org] [DOI] (P p)