CcpN

• Description: transcriptional regulator of the glycolytic genes. repression in the presence of glucose
  
  

Gene name	ccpN
Synonyms	yqzB
Essential	no
Product	transcriptional regulator
Function	repressor of genes involved in gluconeogenesis (gapB, pckA) and of sr1
MW, pI	23.4 kDa, 7.22
Gene length, protein length	636 bp, 212 amino acids
Immediate neighbours	glyS, yqfL
Gene sequence (+200bp)	Protein sequence
Genetic context File:CcpN context.gif

The gene

Basic information

• Coordinates: 2604195 - 2604830

Phenotypes of a mutant

Impaired growth on glucose due to re-routing of carbon from glycolysis to the pentose phosphate pathway PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription repression of the gapB, pckA, and sr1 genes in the presence of glucose PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • YqfL (negative effector of CcpN activity) PubMed
  • ATP enhances CcpN-dependent repression, ADP counteracts the repressing activity of CcpN PubMed

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: [3]

• KEGG entry: [4]

Expression and regulation

• Operon: ccpN-yqfL PubMed

• Sigma factor:

• Regulation: constitutively expressed PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Sabine Brantl, Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany homepage

References

1. Servant et al. (2005) CcpN (YqzB), a novel regulator for CcpA-independent catabolite repression of Bacillus subtilis gluconeogenic genes. Mol. Microbiol. 55: 1435-1451. PubMed
2. Licht et al. (2005) Implication of CcpN in the regulation of a novel untranslated RNA (SR1) in Bacillus subtilis. Mol. Microbiol. 58: 189-206. PubMed
3. Licht & Brantl (2006) Transcriptional repressor CcpN from Bacillus subtilis compensates asymmetric contact distribution by cooperative binding. J. Mol. Biol. 364: 434-448. PubMed
4. Licht et al. (2008) Identification of ligands affecting the activity of the transcriptional repressor CcpN from Bacillus subtilis. J. Mol. Biol. 380: 17-30. PubMed
5. Tännler et al. (2008) CcpN controls central carbon fluxes in Bacillus subtilis. J. Bacteriol. 190: 6178-6187. PubMed